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A study on invasive, or metastatic potential of bladder cancer using a new or thotopic animal model

Research Project

Project/Area Number 06671572
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionUniversity of Tokyo

Principal Investigator

KAMEYAMA Shuji  Univ.of Tokyo, Fac.of Med., Dept.of Urology, Assistant Prof., 医学部(病), 講師 (90186015)

Co-Investigator(Kenkyū-buntansha) FUKUSAWA Ritsu  Univ.of Tokyo, Fac.of Med., Dept.of Urology, Assistant, 医学部(病), 助手 (90251305)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsBladder cancer / invasion / metastasis / 正所性移植 / 動物モデル
Research Abstract

We have established a new in vivo model for studying invasion and metastasis of bladder cancer.The animal model system consisted of a rat bladder transplanted in the retroperitoneum of a nude mouse and connected with a subcutaneous reservoir.The cells we used were rat bladder carcinoma cell lines (LMC19, MYU3L), murine bladder carcinoma cell line (MBT-2), and human transitional cell carcinoma cell lines (SK-S,SK-I). The human cell lines (SK-S,SK-I) were newly establishied from patients with bladder tumor and renal pelvic tumor.In vivo study, SK-S cells formed superficial tumors.MBT-2, MYU3L,and SK-I tumors were invasive, but non-metastatic ones.LMC19 tumors were invasive and metastatic ones.
Overexpression of MMP-2 into MYU3L cells transformed the parental cells (invasive/non-metastatic) to the metastatic phenotype.On the other hand, the metastatic potential was highly suppressed by the introduction of TIMP1, or TIMP2 to LMC19 cells.The expression of carbohydrate antigen was altered between SK-S cells (superficial type) and SK-I cells (invasive type). Reactivity to BSA,ECA,and PNA lectin were increased on the cell surface of SK-I cells.
Our results support the evidence that the invasive or metastatic potential can be closely related with MMP expression and altered expression of carbohydrate antigen.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] S,Kameyama, R.Fukasawa, N.Moriyama,et al.: "A new in vivo model for studying intravesical immunotherapy" J.Urol.153. 485A- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Kawamata, K.Kawai, S.Kameyama,et al.: "Dverexpression of tissue inhibitor of matrix matalloproteinases (TMP1 and TIMP2) suppresses extravasation of pulmonary metastaes of a rat bladder carakoud" Int.J.Cancer. 63. 680-687 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Kawamata, S.Kameyama, K.Kawai,et al: "Marked acceleration of the metastatic phenatype of a rat bleckler carinoma cell line by the expression of human gelatirase A" Int.J.Cancer. 63. 568-575 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 山崎哲,亀山周二,深沢立 他: "ヒト浸潤性膀胱癌細胞株(SKI)の樹立と簡便な同所移植モデルでの性状" 第55回日本癌学期総会記事. 1996. 349- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Kameyama, R.Fukasawa, N.Moriyama, et al.: ""A new in vivo model for studying intravesical immunotherapy"" J.Urol.153. 485A (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Kawamata, K.Kawai., S.Kameyama.et al.: "Overexpression of tissue inhibitor of matrix metallo-proteinases(TIMP1 and TIMP2)Suppresses extravasation of palmonary metastasis of a rat bladder carcinoma"" Int.J.Cancer. 63. 680-687 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Kawamata, S.Kameyama, K.Kawai, et al.: ""Marked acceleration of the metastatic phenotype of a rat bladder caranoma cell line by the expression of human gelatinase A"" Int.J.Cancer. 63. 568-575 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Yamazaki, S.Kameyama, R.Fukasawa, et al.: ""Establishment of a human invasive bladder caranoma cell line(SKI)and its tumorigenicity using a simple orthotopic transplantation model"" Proceedings of the Japanese Cancer Association, 55th Annual Meeting. 349 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 亀山周二,本間久夫,河辺香月,大保亮一: "ラット膀胱を用いた尿路性器癌細胞の浸潤能判定" 第53回日本癌学会総会記事. 370 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 亀山周二,深澤立,森山信男,赤座英之他: "BCG・コンノート株(生菌又は死菌)のマウス膀胱内注入によるサイトカイン誘導能の検討" 第54回日本癌学会総会記事. 431 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kameyama,S,Fukasawa.R,Moriyama,N.et al: "A new in vivo model for studying intravesical immunotherapy" Journal of Urology. 153. 485A (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawamata.H.,Kameyama.S.Kawai.K,Tanaka,Y.,et al: "Marked acceleration of the metustatic phenotype of a rat bladder carcinoma cell line by the expression of human gelatinase A" International Journal of Cancer. 63. 568-575 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawamata.H,Kawai.K.,Kameyama.S,Johnson.M.D,et al: "Over-expression of tissue inhibitor of metrix metalloproteinases (TIMP1 and TIMP2) suppresses extravasation of pulmonary metastasis of a rat bladder carcinoma" Internctional Jounal of Cancer. 63. 680-687 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 亀山周二,深沢立,西松竜明,河辺香月: "C3H/Heマウスを用いた、新しい膀胱癌研究のin vivoモデル" 第82回日本泌尿器科学会総会. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kameyama,S.Fukasawa,R.Moriyama,N.,Kawabe,K: "Some approaches to develop metastatic hone tumor using human prostatic cancer cell lines in a nude mice model" Proceedings of the fifth workshopon on prostate cancer. 14-16 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kameyama,S.,Homma,Y.,Kawabe,K.,Oyasu,R: "In vivo invasion assay of urogenital cancer cells using rat urinary bladders transplanted into nude mice" Proceedings of the Japanese Cancer Association. 370- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kameyama,S.,Fukasawa,R.,Moriyama,N.,Kawabe,K.,Kawai,K: "A new in vivo model for studying intravesical immunotherapy" American Urological Association,1995 Annual meeting.

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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