| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Research Abstract |
Acquired cystic disease of the kidney (ACDK) was frequently observed in patients with a long-term hemodialysis. Both renal cell carcinoma (RCC) and renal cell adenoma were also frequently developed in ACDK patients. In particular, the incidence of papillary RCC in ACDK patients is higher than that in general population. In the present study, chromosome studies in peripheral blood lymphocytes from patients with a long-term hemodialysis revealed the congenital chromosome abnormalities ; del (15q), XYY,t (7q ; 19a) and t (10q ; Xp). It is still unknown whether these congenital chromosome abnormalities are involved in the course of papillary RCC and ACDK development. Chromosome studies were also performed on papillary RCC obtained from five ACDK patients (#121,219,228,235,236) and one case of sporadic papillary RCC (#220), and trisomy of chromosome 16 was identified in 3 cases (#121,219,220) annd trisomy of chromosome 7 in 2 cases (#121,220) respectively. Structural abnormalities involving chromosomes 1,6 and 10 were also observed in 2 cases (#121,219) of ACDK patients. The remaining three cases (#228,235,236) showed a normal karyotype. However, the presence of 7- and 16-trisomies was also confirmed by FISH analyzes using alpha-satellite DNA probes in all of specimens including precancerous ACDK tissues. Although loss of the short ofchromosome 3 was not identified cytogenetically in all of papillary RCC,LOH studies by PCR technique using microsatellite DNA markers on chromosome 3p revealed the reduction of signals at the region of 3p13 in three out of four informative cases.
|
