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Study on effects of insulin-like growth factor on growth of prostate cancer

Research Project

Project/Area Number 06671579
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionToyama Medical and Pharmaceutical University

Principal Investigator

FUSE Hideki  Toyama Mrdical and Pharmaceutical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (40143292)

Co-Investigator(Kenkyū-buntansha) SAKAMOTO Mamoru  Toyama Medical and Pharmaceutical University, Faculty of Medicine, Instructor, 附属病院, 講師 (20178578)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsProstate cancer / Proliferation / Insulin-like growth factor
Research Abstract

Insulin-like growth factor-I (IGF-I) is a potent mitogenic and a differentiation inducing polypeptide growth factor, and is considered to be a significant autocrine-paracrine factor involved in cellular proliferation of normal and malignant tissues. Recently, IGF-I receptors have been characterized in human normal prostate epithelial cells, hyperplastic prostate cells, and rat prostate cancer cells. Furthermore, a family of at least six proteins with high binding affinity for IGFs, the IGF binding proteins (IGFBPs), are described which are different form IGF receptors and are considered to control the proliferative and mitogenic effects of IGFs.
The androgen-dependent prostatic carcinoma cell line, LNCaP-FGC (FGC), its derived androgen-independent sublines, LNCaP-r and LNO,and the androgen-independent cell lines PC-3and TSU-PRI (TSU) were used. The breast cancer cell line MCF-7 cell line served as control cell line for IGF responsiveness. The effects of IGF-I or androgens on the growth … More of the cells were assessed by the use of a test based on the enzymatic reduction of the tetrazolium salt MTT.Conditioned medium proteins were separated on sodium dodecylsulfate-polyacrylamide gel and transferred to nitrocellulose paper and IGFBPs were detected by Western blotting. IGF-I recceptor content of membrane preparated was determined by saturation analysis. The data were calculated by multiple-point Scatchard analyzes.
IGF-I stimulated MCF-7 cells dose-dependently (1-100ng/ml). Under the same conditions FGC,LNCaP-r, LNO,and PC-3 cell lines did not respond to IGF-I,whereas it slightly stimulated growth of the TSU line. Androgen addition to IGF-I did not influence the cell growth of the andfrogen-dependent FGC cell line. High affinity IGF-I receptors were demonstrated in all cell lines lested, the receptor content of the FGC line being lower than the other androgen-independent cell lines. All prostatic cancercell lines secreted IGFBP-2, whereas IGFBP-3 and/or IGFBP-4 were also detected in the medium of LNCaP-r, PC-3 and TSU cultures.
In spite of the presence of high affinity IGF receptors, with one exception (TSU), IGF-I had no effect on cell proliferation of the prostatic cancer cell lines tested. As has been described for other tissues, IGFBP-2 may be a factor that inhibits the biological activity of IGF-I in these cell lines, whereas some other IGFBPs, such as IGFBP-4 may enhance IGF-I responsiveness of the cells. It is therefore suggested that IGFBPs regulate IGF-I mediated cell proliferation of prostate cancer cells. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Fuse,H.et al.: "Effects of insulin-like growth facter-I on growth of humen prostrte Cancer all lines" Proceeding of the Fifth workshop on Prostrte cancer. 27-29 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuse,H.et al.: "Bone merrow scintiguphy in the diagrosis of bone metastisis in prostnte cancer" International Urology and Nephrology. 26. 53-61 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuse,H.et al.: "Epiderwol growth facter concentrations in seminal plesma from pafients with by pogoradism-Chauges after hormore replacement" Urologir Intertionalis. 52. 9-13 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuse,H.et al.: "Clinicl study on prognosis of metastatic prostate cancer lmsed on estet of diseare on pretrectment bone scintigmphy" International Urology and Nephrology. 27. 567-574 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 酒本護他: "富山県細入村における過去9回の前立腺集団検診の検討" 腎泌予防医誌. 3. 68-69 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuse, H.et al.: "Effects of insulin-like growth factor-I on growth of human prostate cancer cell lines." Proceedings of the Fifth Workshop on Prostate cancer. 27-29 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuse, H.et al.: "Bone marrow scintigraphy in the diagnosis of bone metastasis in prostate cancer." Intermationl Urology and Nephrology. 26. 53-61 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuse, H.et al.: "Epidermal growth factor concentrations in seminal plasma from patients with hypogonadism." Urologia Internationalis. 52. 9-13 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuse, H.et al.: "Correlation of seminal plasma transferrin concentration and hypoosmotic swelling of the sperm." Archives of Andrology. 32. 95-100 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuse, H.et al.: "Clinical study on prognosis of metastatic prostate cancer based on extent of disease on pretreatment bone scintigraphy." International Urology and Nephrology. 27. 567-574 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuse,H et al.: "Effects of insulin-like growth factor-I on growth of human cancer cell lines" Proceedings of the Fifth workshop on Prostate cancer. 27-29 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fuse,H et al.: "Bone marrow scintigraphy in the diagnosis of bone metastasis in prostate cancer" International Urology and Nephrology. 26. 53-61 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fuse,H et al.: "Epidermal growth factor concentrations in seminal plasma from patients with hypogonadism" Urologia Internationalis. 52. 9-13 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fuse,H et al.: "Correlation of seminal plasma transferrin concentration and hypoosmotic swelling of the sperm" Archives of Andrology. 32. 95-100 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fuse,H et al.: "Clinical study on prognosis of metastatic prostate cancer based on extent of disease on pretreatment bone scintigraphy" International Urology and Nephrology. 27. 567-574 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 酒本 護: "富山県細入村における過去9回の前立腺集団検診の検討" 腎泌予防医誌. 3. 68-69 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fuse,H.et al.: "Effects of insulin-like growth factor-I on growth of fuman prostate cancer cell lines" Proceedings of the Fyth workshop on Pratrte cancen. 27-29 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fuse,H.et al.: "Epideranl growth factor concentations on semiral plauna from pifents with hypogonadis-Chages after hotmone refaconen" urologia Internatioalis. 52. 9-13 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fuse,H.et al.: "Correlation of Seminal plarna transferrin concentrtion and hypoosmofic swellig Hthe sperm" Archives of Andrology. 32. 95-100 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fuse,H.et al.: "Bcne marron scintigmphy in the diagnosis of bone netstasis in prostete cancer" International Urology and Nephrology. 26. 53-61 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Iwasaki,M.,Fuse,H.et al.: "Histologicelard Cndocrinological in vextrgations of Cuclosporine effeits on the raro testis" Andrologia. 27(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fuse,H.et al.: "Year Book of Infevtiloq and Reproductive Endocrinology" Mishell,D.R.Jr.,Lobo,R.A.,Sokol,R.Z., 333 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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