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MECHANISM OF ANDROGEN DEPENDENT MORPHOGENESIS OF PROSTATE GLAND : ROLE OF GROWTH FACTORS IN ORGAN CULTURE.

Research Project

Project/Area Number 06671583
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionAICHI CANCER CENTER

Principal Investigator

SUGIMURA Yoshiki  AICHI CANCER CENTER,RESEARCH FELLOW, 研究所, 研究員 (90179151)

Co-Investigator(Kenkyū-buntansha) HIOKI Takuichi  DEPARTMENT OF UROLOGY,AICHI CANCER CENTER,CLINICAL STAFF, 泌尿器科部, 医長
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsPROSTATE / ORGAN CULTURE / ANDROGEN / GROWTH FACTOR / DUCTAL MORHOGENESIS / KGF / EGF / 無血清器官培養法 / 形態測定
Research Abstract

To investigate the androgenic signal communicating from androgenreceptor-positive mesenchyme to the epithelium, the functions of keratinocyte growth factor (KGF) on the prostate branching morphogenesis was examined with serum-free prostate organ culture system in the rat ventral prostate (VP) gland. Organ culture of rat neonatal VP's were incubated on the Millipore filters under serum and growth factor-free conditions. When testosterone (T,10^<-8>M) was added to the basal media, VP's grew and underwent branching morphogenesis similar to that in situ. Neutralizing antibody to KGF,inhibited androgen-stimulated VP growth after 6 days in culture. When KGF was added to the media instead of T,it stimulated VP growth to a levels of 50% in branching ends of that which is possible with an optimal dose of Testosterone. KGF is a member of the fibroblast growth factor family and is suggested to be produced by mesemchymal/fibroblast cells and is able to act sole on epithelial cells. In this study.we demonstrated that KGF play an important role as a paracrine mediator on the mesenchymal-epithelial interaction in the branching morphogenesis required for prostate development.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 杉村芳樹: "前立腺の発生:アンドロゲン依存性と上皮間質の相互作用" 臨床泌尿器科. 49. 7-16 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 杉村芳樹: "前立腺の増殖性疾患:肥大症と癌" 現代医学. 42. 259-263 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cunha GR, Sugimura Y. et al: "The role of growth factors in the development of the prostate and seminal vesicle" Biomed & Pharmacother. 48. 9s-17s (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cunha GR et al: "Growth factors as mediators of androgen action during the development of the male mogenital forct." World J. Urol. 13. 264-276 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yonemura, C・Y et al: "Temporal and Spatiol factors in diethylstilbestrol-induced sruamous metaplasia in the developing human prostate II" Acta Anat. 153. 1-11 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 杉村芳樹: "前立腺肥大症自然史の基礎:前立腺増殖における生物学的特性" 日泌尿会誌. 88(印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugimura. Y. et al.: "Mesenchymal-epithelial interaction in Prostate growth in “Foundemeatul Approthes to the Diujnosis and treetment of Prostote Cancer and BPH"" Kyoichi Imai, Jun Shimazaki, Jamis P karr(eds). Adenin Press, 6 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cunha GR & Sugimura Y: "The role of growth factors in the development and growth of the Proste and Seminal Vesicle in "Growth Factor and B. P. M" M-C Andro, (ed), Elsevier press (in press), (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cunha GR,Sugimura Y,Foster B,Rubin JS,Aaronson SA: "The role of growth factors in the development and growth of the prostate and seminal vesicle. In : Growth Factors and BPH,M-C Andro, ed." Elsevier, New York. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugimura Y: "Basic study in the natural history of benign prostatic hyperplasia : biology of prostatic growth." Jap J Urology. (in press). (1966)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cunha GR,Foster B,Thompson A,Sugimura Y,Tanji N,Tsuji M,Terada N,Finch P,Donjacour AA: "Growth factors as mediators of androgen action during the development of the male urogenital tract." World J Urol.13. 264-276 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yonemura CY,Cunha GR,Sugimura Y,Mee SL: "Temporal and spatial factors in diethylstilbestrol-induced squamous metaplasia in the developing human prostate : II.Persistent changes after removal of diethylstilbestrol" Acta Anatomica. 153. 1-11 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cunha GR,Sugimura Y,Foster B,Rubin JS,Aaronson SA: "The role of growth factors in the development and growth of the prostate and seminal vesicle." Biomed & Pharmacother. 48, Suppl 1. 9s-17s (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugimura Y: "Developmental biology of prostate gland : Androgen dependency and epithelial-mesenchymal interaction." Jap J Clin Urol. 49. 7-16 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugimura Y,Hioki T,Hayashi N,Kawamura J,Rubin JS,Aaronson SA,Cunha GR: Mesenchymal-epithelial interactions in prostate growth : Role of growth factors as androgenic signals. in Imai, K., Shimazaki, J., Karr, J.P.(eds) : Fundametal approaches to the Diagnosis and Treatment of Prostate Cancer and BPH. New York, Adenine Press, 97-102 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cunha GR,Foster B,Donjacour AA,Rubin JS,Sugimura Y,Finch PW,Aaronson SA: Keratinocyte growth factor : a mediator of mesenchymal-epithelial interactions in the development of androgen target organs. M Motta and M Serio (eds) : Sex hormones and antihormones in endocrine dependent pathology : basic and clinical aspects.Elsevier Science B.V., 45-57 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cunha GR, et al: "Grouth factors as mediators of androgen action during the development of the male urogevitre torct" World J Uvol. 13. 264-276 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yonemura C. Y. et al: "Temporal and Spatial factors in dietylstilbestrol-induced squamous metuylaria in the developing human proctate II." Acta Anrt. 153. 1-11 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Cunha GR, Sugimura Y. et al: "The role of grouth factors in the development and grouth of the prostote and reminal Jesicle" Bioned & Prarmacother. 48. 9s-17s (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Cunha GR, Sugimura Y: "Therole of growth factors in the development and growth of the prostate and seminal vesicle, in: Growth factors and BPH." M-C Andro, ed. Elsevier, New York(in press), (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 杉村芳樹: "前立腺の発生:アンドロゲン依存性と上皮・間質の相互作用" 臨床泌尿器科. 49. 7-16 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 杉村芳樹: "(臨床と研究)〃前立腺の増殖性疾患 肥大症と癌〃" 現代医学(愛知県医師会誌). 42(印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sugimura Y.: "Found a mental Approaches to the Diagnasis and Treatment of Prostates Cancer,and Bpll.“Mesencly,mal-Epitholial Initeraction in Prostaric,Growth"" Kyoichi Imai,Jan Shimazaki,James P.Karr eds,Adenine Press, 6 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Canha,GR.: "Sex hormores and antihor mors in endocrinc dependent pathology “keratino syte growth factor"" Motta M,Serio Mods,Elseuier Science, 13 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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