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Basic study on the mechanism of intralaminal seeding of urothelial tumor using a short-term culture system for exfoliuted cells in patients urine

Research Project

Project/Area Number 06671586
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KAKEHI Yoshiyuki  Kyoto University, Faculty of Medicine, Assistant Professor, 医学研究科, 講師 (20214273)

Co-Investigator(Kenkyū-buntansha) MIZUTANI Youichi  Kyoto University, Faculty of Medicine, Lecturer, 医学研究科, 助手 (10243031)
OGAWA Osamu  Kyoto University, Faculty of Medicine, Lecturer, 医学研究科, 助手 (90260611)
TERACHI Toshiro  Kyoto University, Faculty of Medicine, Assistant Professor, 医学研究科, 講師 (50207487)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsurothelial cancer / urinary exfoliated cell / intraluminal seeding / p53 / molecular diagnosis / 尿路上皮腫瘍 / 腔内播種 / 短期培養 / p53免疫細胞染色
Research Abstract

We have tried to cultivate urinary exfoliated cells and to elucidate the mechanism of the intraluminal seeding of urothelial tumor cells. The sucess rates of both primary cell outgrowth and the first passage for urine cells from urothelial cancer patients were 87% and 75%, respectively, which were significantly higher than those for healthy volunteers (62% and 35%, respectively). The success rate of this short-term culture was not correlated with tumor grade or stage. Interestingly, the success rates for primary growth and the first passage were 100% for urine cells from the patients with upper urinary tract tumor, while those from bladder tumor patients were 65% (p<0.05). The success rates for urine cell culture were correlated with the p53 mutational status in tumor cells. The success rate for patients with p53 positive tumors was 85%, while that for patients with p53 negative tumors was 58% (p<0.05). The results of the immunocytochemical diagnosis for p53 status for urine cultured cells were highly identical (92.1%) with the results of the imunohistochemical staining of p53 for tumor cells in each patient. We have succeeded in detecting the same p53 gene mutation as the corresponding tumor in the urine culcure cell-derived DNA by the mutant allele-specific amplification.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report

Research Products

(28 results)

All Other

All Publications (28 results)

  • [Publications] 川喜多睦司: "尿路上皮癌におけるE-カドヘリンの発現と浸潤・転移の検討" 泌尿器科紀要. 40. 1077-1080 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 筧善行: "癌抑制遺伝子解析の臨床的意義" 泌尿器外科. 8. 527-533 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 赤尾利弥: "II. 分子生物学の病理診断への応用、B.病理・病態診断への将来の展望 I、腫瘍および類似疾患(2)臍肢癌" 病理と臨床. 14. (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tomonori Habuchi: "Oncogene Amplification in Urothelial Cancers with p53 Gene Mutation or MDM2 Amplification" Journal of National Cancer Institute. 86. 1331-1335 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshiyuki Kakehi: "Comparison of multidrug resistance gene expression levels with malignant potentials and influence of dremotherapy in Urothelial Cancers" International Journal of Urology. 2. 309-315 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wen-Jeng Wu: "Allelic Frequency of p53 Gene Coden 72 Polymorphisen in Urologic Cancers" Japanese Journal of Cancer Research. 86. 730-736 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Keiji Ogura: "Immunohistochemical analysis of p53 and proliferating cell naclear antigen (PCNA) in blodoler cancer : positive immunostaining and radiosensitivity" International Journal of Urology. 2. 300-308 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Osamu Yoshida: "Recent advances in the molecular genetics of urogenital tumors" International Journal of Urology. 1. 1-16 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Youichi Mizutani: "In vitro enhancement of natural Killer cell activity and the antagonistic inhibition of the susceptibility of K562 cells to lysis..." International journal of Urology. 1. 49-56 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Youichi Mizutani: "Enhancement of sensitivity of urinary bladder tumor cells to cosplatin by c-myc antisense digonucleotide" Cancer. 74. 2546-2554 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Youichi Mizutani: "Enhancecl antitumor effect of Bacillus Culmette Guerin in combinetiron with fibrinogor on urinary bladder tuncor" Journal of Urology. 151. 1420-1426 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Youichi Mizutani: "Seram gronulocyte colony-stimulating factor levels in patients with urinary bladder tumor and various" British Journal of Urology. 76. 580-586 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mutsushi Kawakita, seiji Moroi, Kyoko Kyotani, Yoshiyuki Kakehi, Osamu Yoshida: "Study on E-cecherin expression in relation to invasion and metastasis in urothelial cancerpatients" Acta Urol Jpn. 40. 1077-1080 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Osamu Yoshida, Tomonori Habuchi and Osamu Ogawa: "RECENT ADVANCES IN THE MOLECULAR GENETICS OF UROGENITAL TUMORS" Int J Urol. 1. 1-16 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Youichi Mizutani and Osamu Yoshida: "In Vitro Enhancement of Natural Killer Cell Activity by BCG and the Antagonistic Inhibition of the Susceptibility of K562 Cells to Lysis by Peripheral Blood Lymphocytes in Patients with Urinary Bladder Tumor" Int J Urol. 1. 49-56 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tomonori Habuchi, Hidefumi Kinoshita, Hitoshi Yamaca, Yoshiyuki Kakehi and Osamu Yoshida: "Oncogene Amplification in Urothelial Cancers With p53 Gene Mutation or MDM2 Amplification" J.Natl Cancer Inst. 86. 1331-1335 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Youichi Mizutani, Manabu Fukumoto, Benjamin Bonavica and Osamu Yoshida: "Enhancement of Sensitivity of Urinary Bladder Tumor Cells to Cisplatin by c-myc Antisense Oligonucleotide" Cancer. 74. 2546-2554 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Youichi Mizutani, Manabu Fukumoto and Osamu Yoshida: "Enhanced Antitumor Effect of Bacillus Calmette-Guerin in Combination With Fibrinogen on Urinary Bladder Tumor" J.Urol. 151. 1420-1426 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshiyuki Kakehi, Wen-Jen Wu, Wun-Jae Kim, Shinji Arao, Manabu Fukumoto and Osamu Yoshida: "Comparison of multicrug resistance gene expression levels with malignant potentials and influence of chemotherapy in urothelial cancers" Int J Urol. 2. 309-315 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Youichi Mizutani, Yusaku Okada, Toshiro Terachi, Yoshiyuki Kakehi and Osamu Yoshida: "Serum granulocyte colony-stimulating factor levels in patients with urinary bladdertumor and various urological malignancies" Br.J.Urol.76. 580-586 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wen-Jeng Wu, Yoshiyuki Kakehi, Tomonori Habuchi, Hidefumi Kinoshita, Osamu Ogawa, Toshiro Terachi, Chun-Hsiung Huang, Chin-Pei Chiang and Yoshida O.: "Allelic Frequency of p53 Gene Codon 72 Polymorphism in Urologic Cancers" Jpn.J.Cancer Res.86. 730-736 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] KEIJI OGURA,HITOSHI YAMADA,OSAMU OGAWA AND OSAMU YOSHIDA: "IMMUNOHISTOCHEMICAL ANALYSIS OF P53 AND PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) IN BALDDER CANCER : POSITIVE IMMUNOSTAINING AND RADIOSENSITIVITY" Int J Urol. 2. 302-308 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshiyuki Kakehi: "Clinical Implication of the analysis of the tumor Supressor genes" Hinyoki GeKa. 8(7). 527-533 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshiya Akao, Yoshiyuki Kakehi, Osamu Yoshida: "II.Application of molecular biology to pathological Diagnosis. 12)Bladder Cancer" Byori to Rinsho. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 川喜田睦司ら: "尿路上皮癌におけるE-カドヘリンの発現と浸潤・転移の検討" 泌尿器科紀要. 40. 1077-1080 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 筧 善行: "尿路性器癌における癌抑制遺伝子解析の進歩:癌抑制遺伝子解析の臨床的意義" 泌尿器外科. 8. 527-533 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 赤尾利弥: "A.分子生物学の病理診断への応用 B.病理・病態診断への将来の展望 1.腫瘍および類似疾患 127膀胱癌" 病理と臨床. 14. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 川喜田睦司: "尿路上皮癌におけるE-カドヘリンの発現と浸潤・転移の検討" 泌尿紀要. 40. 1077-1080 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-03-31   Modified: 2016-04-21  

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