|Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Gene cloning of bacterial pili of Eschrichia coli or Serratia marcescens was performed, and effect of structure of adhesine and bacterial pili on the experimental pyelonephritis was investigated. The adhesine on the MS-piliated bacteria of Eschrichia coli and Serratia marcescens stringly stimulated scar formation following pyelonephritis, suggesting that the MS-adhesin stimulated polymorhonuclearleukocytes (PMNs) in the infected site, and PMNs released active oxygen speceis resulting tissue injury arround renal tissue. Renal scarring following infection with MS-piliated bacteria was suppressed by scavengers of superoxide such as CV3611, Ebselen, Steroid and ulinastatin. These scavengers were effective at the combination of antimicrobial agents.
PMN-function was suppressed in the renal medulla due to hyperosmolality. The mechanism of suppression of PMNs were thought to be ATP exhausion at Na or direct inactivation at urea. Ofloxacin and fleroxacin, new quinolone antimicrobials, enhanced PMN-function even in hyperosmotic condition, suggesting that these agents were more effective in the treatment of pyelonephritis.