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The role of gap junctional intercellular communication in renal tubular cell proliferation

Research Project

Project/Area Number 06671598
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionNagasaki University

Principal Investigator

NOMATA Koichiro  NAGASAKI UNIV., SCHOOL OF MED., Hospital, INSTRUCTOR, 医学部附属病院, 講師 (80189430)

Co-Investigator(Kenkyū-buntansha) KANETAKE Hiroshi  NAGASAKI UNIV., SCHOOL OF MED., ASSISTANT PROFESSOR, 医学部, 助教授 (50100839)
SAITO Yutaka  NAGASAKI UNIV., SCHOOL OF MED., PROFESSOR, 医学部, 教授 (70039832)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsRenal tubular cell / Growth factor / Renal carcinogen / Cell cell communication / Renal cell carcinoma / ギャップ結合
Research Abstract

Gap junctional intercellular communications (GJIC) are known as the channels for the direct transfer of cytoplasmic molecules between neighboring cells and are lost during transformation of normal cells. To study the function, and the molecular mechanism for the loss of GJIC,the effects of dimethylnitrosamine, KBrO_3, FeSO_4・7H_2O,which are known as chemical tumor promoters of the kidney on the GJIC function and the expression of connexin 43 of Madin-Darby Canine Kidney (MDCK) cells were examined. These tumor promoters inhibited the GJIC in MDCK cells. The expression of connexin 43 mRNA and connexin 43 protein were not altered by these treatment, whereas immunohistochemical study revealed that the distribution of connexin 43 protein was changed from the cell surface to the cytoplasma. These data suggest that blockage of GJIC in MDCK cells treated with renal carcinogens support the hypothesis that loss of GJIC might be important in renal carcinogenesis.
Freeze-fracture replicas were prepared from the operative specimens of 14 patients with renal cell carcinoma. Gap junctions in the renal cell carcinoma (RCC) tissues were significantly fewer than in the surrounding normal tissue, as for the frequency of gap junctions there was no obvious relation in tumor grade and pathological stage. However, there is no clearly difference in freqency of tight junctions between RCC tissues and surroungins normal tissues. These data suggest that the loss of intercellular communication via gap junction may be associated with renal cell carcinoma formation.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] K.Nomata, et al: "Renal cell carcinoma and angiogenic factors." Kidney. 6(4). 141-142 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Kanda, et al: "Activity of keratinocyte growth factor-like substance extracted from rabbit liver on renal tubular cell growth during compensatory renal hyperplasia." International Journal of Urology. 4. 380-387 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nomata, et al: "Freeze-fracture studies of gap junction in human renal tissue with special reference to their relationship to renal cell carcinoma and surrounding normal renal tissue." British Journal of Urology. 80. 117- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Noguchi, et al: "The role of gap junctional intercellular communication in renal cell carcinogenesis." British Journal of Urology. 80. 126- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Noguchi, et al: "Changes in the gap junctional intercellular communication in renal tubular epithelial cells in vitro treated with renal carcinogens." Cancer Letters. 122. 77-84 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nomata, et al: "Renal cell carcinoma and angiogenic factors." Kidney. 6 (4). 141-142 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Kanda, et al: "Activity of keratinocyte growth factor-like substance extracted from rabbit liver on renal tubular cell growth during compensatory renal hyperplasia." International J.Urology. 4. 380-387 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nomata, et al: "Freeze-fracture studies of gap junction in human renal tissue with special reference to their relationship to renal cell carcinoma and surrounding normal renal tissue." British J.Urology. 80. 117 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Noguchi, et al: "The role of gap junctional intercellular communication in renal cell carcinogenesis." British J.Urology. 80. 126 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Noguchi, et al: "Changes in the gap junctional intercellular communication in renal tubular epithelial cells in vitro treated with renal carcinogens." Cancer Letters. 122. 77-84 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nomata.et al.: "Inhibition of gap-junctional intercelluar communication in hepathchlor and hpatachlor epoxide-treated normal human breast epithelial cells." Cell Biology and Toxicology. 12(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] C-Ykao,K.Nomata,et al: "Two types of normal human breast epithelial cells derived from reduction mammoplasty : Phemotypic characterization and response to SV40 transfection" Carcinogenesis. 16(in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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