| Project/Area Number |
06671628
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Chiba University |
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Principal Investigator |
OSADA Hisao Chiba Jniversity School of Medicine, Assistant, 医学部, 助手 (30233505)
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| Co-Investigator(Kenkyū-buntansha) |
SEKIYA Souei Chiba University School of Medicine, Professor, 医学部, 教授 (00092065)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Pregnancy / Major histocompatibility complex (MHC) / Placenta / Trophoblast / Transgenic mice |
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| Research Abstract |
In this study we attempted to investigate directly the significance of regulation in major histocompatibility complex (MHC) antigen expression as a mechanism required for successful pregnancy. Transgenic mice expressing MHC antigens on trophoblasts of the placenta were produced to inquire whether transgenic fetuses are rejected by their mother or not.
The chimerical genes were constucted from both the regulatory region of human c-fos or b-actin gene, which can be expressed on the placenta, and the cording region of H-2K^b gene. A microinjection of these constructed genes. produced 30 founder mice. H-2K^b antigen expression was detected on peripheral nucleated cells of 9 founder mice. However, the mating between their males and H-2K^b negative females resulted in a similar number of the transgene-positive and-negative F1 mice without symptoms indicating the rejection of feuses.
There is the possibility that a lack of transgene expression on trophoblasts led to no rejection of transgenic fetuses by their mothers. Therefore, placentas together with fetuses were resected and subjected to a histological staining with anti-H-2K^b antibody. No H-2K^b antigens were detected on the placental trophoblasts of transgene-positive fetuses. Additionally, in situ primer extension analysis using H-2K^b specific scquence failed to show a presence of H-2K^b mRNA.
Human chorionic gonadotropin (hCG) is the pregnancy-specific hormone synthesized and secreted by trophoblast cells from early pregnancy period. A new chimerical genc was thus constructed from the regulatory region of 5' side of hCG-beta gene and the cording region of H-2K^b gene. The production of transgenic mice using this chimerical gene is in progress. We schedule the screening analyzes described above for these mice.
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