INHIBITORY EFFECT OF ANGIOGENESIS INHIBITOR TNP-470 ON MALIGNANT TUMOR

Research Project

Project/Area Number	06671634
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Obstetrics and gynecology
Research Institution	NIIGATA UNIVERSITY
Principal Investigator	KODAMA Shoji NIIGATA UNIVERSITY,GYNECOLOGY AND OBSTETRICS ASSISTANT PROFESSOR, 医学部, 助教授 (50205415)
Co-Investigator(Kenkyū-buntansha)	田村正毅新潟大学, 医学部・附属病院, 助手 (20262446)
Project Period (FY)	1994 – 1995
Project Status	Completed (Fiscal Year 1995)
Budget Amount *help	¥1,400,000 (Direct Cost: ¥1,400,000) Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000) Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Keywords	Antitumor activity of angiogenesis inhibitor / TNP-470 / がん細胞抑制
Research Abstract	Antitumor activity of angigenesis inhibitor TNP-470 was evaluated in 9 human cultured cell lines. Direct effect of TNP-470 was observed by [^3H] concentration in 9 cell lines cultured with [^3H] TNP-470. Although total [^3] concentration showed no significant differences in earch cell lines, the inhibitory percentages by TNP-470 were more dominant from 11.0 to 46.7% in strongly inhibited cell lines than from 2.3 to 4.4% in poorly inhibited cell lines. The antitumor effective mechanism of this compound was studied in these cell lines measured by concentration of [^3H] thymidine, [^3H] uridine, and [^3H] leucine after administration of TNP-470. The most dominant inhibition time in these cell lines was 36 hours after administration of this compound. At this time, percentages of inhibition with [^3H] thymidine were significantly dominant from 64% to 88% than that with [^3H] uridine, and [^3H] leucine after administration of this compound. These results suggest that the antitumor effective mechanism of this compound is inhibition of DNA sythesis in tumor cells.