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Moleccular analysis of human leukocyto antigens in trophblast derived cells

Research Project

Project/Area Number 06671650
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionOsaka University

Principal Investigator

KOYAMA Masayasu  Osaka Univ.Med.School Dept.of Obstet.and Gyne., research assistant, 医学部, 助手 (00183351)

Co-Investigator(Kenkyū-buntansha) KAMIURA Shoji  Osaka Univ.Med.School Dept.of Obstet.and Gyne., research assistant, 医学部, 助手 (10243213)
OHASHI Kazutomo  Osaka Univ.Med.School Dept.of Obstet.and Gyne., research assistant, 医学部, 助手 (30203897)
AZUMA Chihiro  Osaka Univ.Med.School Dept.of Obstet.and Gyne., research assistant, 医学部, 助手 (20151061)
SAJI Fumitaka  Osaka Univ.Med.School Dept.of Obstet.and Gyne., assistnt professor, 医学部, 講師 (90093418)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsMajor histocompatibility / HLA-G / Choriocarcimoma / nuclear factor / NF-kappaB / Gel mobility shift assay / trophoblast / 免疫学的妊娠維持機溝 / 転写因子
Research Abstract

Mammalian conceptus, which is immunologically semi-allogeneic to the mother, can grow and develop in the mother's uterus avoiding rejection by a component maternal immune system during pregnancy. The molecules on the allogencic fetus and chorionic tissue that arc most active in eliciting an antibody response in the mother arc class 1 major histocompatibility complex (MHC) antigens. We employed several trophoblast derived cell lines to clarify the mechanism regulating the expression of HLA class 1 mRNA at transcriptional level. Northern blot hybridization probed classical HLA class I gene (HLA-A) and nonclassical one (HLA-G) could show the heterogeneity of the expression of HLA class 1 genes among trophoblast cell lines. Using trophoblastic cell lines which express classical HLA class 1, we could demonstrate the nuclear protein that binds the promoter region (enhancer-A) and the expression of this nuclear protein was stimulated by the addition of interfcron-g (published in Am.J.Reprod.Immunol.34 : 323-331 1995). Furthermore, the interaction between nuclear protein and enhancer region was inhibited by anti-NF-kB p5O antibody indicating that the NF-KB p5O protein may play an important regulator to express classical HLA class 1 in trophoblast. On the other hand, we could show that the novel nuclear protein distict from NF-kB protein in the trophoblastic cell line, BeWo, recognizes the specific region located at the promoter region of HLA-G gene (data are submitted in academic journal). Characterization of this novel protein are now on going.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] A. Wakimoto,et al.: "Regulation of Classical HLA Class I Genes in Human Choriocarcinoma Cells by Nuclear Proteins Binding to MHC Class I Regulatory Elements" American Journal of Reproductive Immunology. 34. 323-331 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Hashimoto,et al.: "Loss of Imprinting in Choriocarcinoma" Nature Genetics. 9. 109-110 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Sawai,et al.: "Leukemia Inhibitory Factor (LIF) Enhances Trophoblast Differentiation Mediated by Human Chorionic Gonadotropin (hCG)" Biochemical and Biophysical Research Communications. 211. 137-143 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Kikuchi,et al.: "Loss of Biologidal Activity of Human Chorionic Gonadotropin (hCG) by the Amino Acid Substitution on the “CMGCC" Region of the Alpha Subunit" Molecular and Cellular Endocrinology. 102. 1-7 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 古山將康,他: "絨毛癌由来細胞株からの核蛋白と非古典的クラスI遺伝子上流域との特異的結合" 日本組織適合性学会誌. 1. 31-32 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Kimura,et al.: "Binding of Nuclear Factors derived from Choriocarcinoma Cell Lines to Non-classical HLA Class I Genes,HLA-G" Proceeding of the 8th Annual Meeting Japan Society for Immunology of Reproduction. 201-204 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Akinori Wakimoto et al.: "Regulation of classical HLA class 1 genes in human choriocarcinoma cell by nuclear proteins binding to NHC class 1 regulatory elements" American Journal of Reproductive Immunology. 34. 323-331 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazumasa Hashimoto et al.: "Loss of imprinting in choriocarcinoma" Nature Geenetics. 9. 109-110 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Keisuke Sawai et al.: "Leukemia inhibitory factor(LIF) enhances trophoblast differentiation mediated by human chorionic gonadotropin(hCG)" Biochemical and Biophysical Research Communications. 211. 137-143 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tomoyuki Kikuchi et al.: "Loss of biological activity of human chorionic gonadotropin(hCG) by the amino acid substitution on the "CMGCC" region of the alpha subnit" Molecular and Cellular Endocrinology. 102. 1-7 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Kimura et al.: "Binding of nuclear factors derived from choriocarcinoma cell lines to non-classical HLA class 1 genes, HLA-G" Proceeding of the 8th Annual Meeting Japan Society for Immunology Reproduction. 210-204 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Wakimoto et al.: "Regulation of Classical HLA Class 1 Genes in Human Choriocarcinoma Cells by Nuclear Proteins Binding to MHC Class1 Regulatory Elements" American Journal of Reproductive Immunology. 34. 323-331 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Hashimoto et al.: "Loss of Imprinting in Choriocarcinoma" Nature Genetics. 9. 109-110 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Sawai et al.: "Leukemia Inhibitory Factor (LIF) Enhances Trophoblast Differentiation Mediated by Human Chorionic Gonadotropin (hCG)" Biochemical and Biophysical Research Communications. 211. 137-143 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Kikuchi et al.: "Loss of Biologidal Activity of Human Chorionic Gonadotropin (hCG) by the Amino Acid Substitution on the “CMGCC" Region of the Alpha Subunit" Molecular and Cellular Endocrinology. 102. 1-7 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 古山將康 他: "絨毛癌由来細胞株からの核蛋白と非古典的クラスI遺伝子上流域との特異的結合" 日本組織適合性学会誌. 1. 31-32 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Kimura et al.: "Binding of Nuclear Factors derived from Choriocarcinoma Cell Lines to Non-classical HLA ClassI Genes,HLA-G" Proceeding of the 8th Annual Meeting Japan Society for Immunology of Reproduction. 201-204 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fumitaka Saji et al: "Current Topic:Human placental receptors." Placenta. 15. 453-466 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tomoyuki Kikuchi et al.: "Loss of biological activity of human chorionic gonadotrop (hCG)by the amino acid substitution “CMGCC"region of α subun" Mol.Cell.Endocrinology. 102. 1-7 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] The recurrent miscarriage immunotherapy trialists group: "World wide collaborative observational study and meta-analysis on allogenic leukicyte immunotherapy for recurrent spontaneous abortion" Am.J.of Reproduct.Immunol.32. 55-72 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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