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Research for the effects of various cytokines on the growth and the invasiveness of ovarian cancer

Research Project

Project/Area Number 06671665
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKyushu University

Principal Investigator

KOBAYASHI Hiroaki  Kyushu Univ., Faculty of Medicine, Lecturer, 医学部, 助手 (70260700)

Co-Investigator(Kenkyū-buntansha) OKADOME Masao  Kyushu Univ., Faculty of Medicine, Lecturer, 医学部, 助手 (30260699)
SAITO Toshiaki  Kyushu Univ., Faculty of Medicine, Senior Lecturer, 医学部, 講師 (80162212)
KAMURA Toshiharu  Kyushu Univ., Faculty of Medicine, Associate Professor, 医学部, 助教授 (30152870)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsOvarian cancer / Interleukin-6 / Antisense Oligonucleotide / Autocrine Growth Factor
Research Abstract

Interleukin-6 (IL-6) is a multifunctional cytokine which has been reoently reported to be produced by human ovarian cancer. To evaluate the effects of IL-6 on the growth of ovarian cancer, we used 11 human ovarian cancer cell lines and got the results as follows :
1.All of 10 epithelial-originated ovarian cancer cell lines produced bioactive IL-6. On the other hand, one ovarian cancer cell line of non-epithelial-origin did not release detectable IL-6 into its culturemedium.
2.Antisense oligonucleotide for IL-6 specifically inhibited both cell growth and the IL-6-production of all IL-6-producing ovarian cancer cell lines. The growth inhibitory effect of the antisense oligonucleotide could be diminished not the co-administration of exogenous IL-6 but by the co-administration of IL-6-sense oligonucleotide.
3.The specific growth inhibitory activity of IL-6-antisense oligonucleotide was not be observed in the case of IL-6-non-producing ovarian cancer cell line.
4.The growth potential of all ovarian cancer cell lines was affected by neither recombinant human IL-6 nor its neutralizing antibody.
5.By the analysis for gene expression of IL-6 receptor and gp130 signal transducer, four of ten IL-6-producing ovarian cancer cell lines seemed to possess the ability to mediate the signal of extracellular IL-6. The other six Il-6-producing cell lines and an IL-6-non-producing cell line did not express IL-6 receptor.
These results suggest that endogenous IL-6 produced by ovarian cancer cells may behave as a growth stimulator by an intracellular autocrine mechanism not involving a mechanism through the cell membrane, although further studies are required to verify this hypothesis.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] T. Saito, et al.: "Antiproliferative lymphokine production by human peripheral blood lymphocytes and lymph node lymphocytes detected by a modified double layer soft agarose clonogenic assay" Lymphokine and Cytokine Research. 13. 55-62 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H. Kobayashi, et al.: "Variant sublines of early-stage human melanomas selected for tumorigenicity in nude mice express a multicytokine-resistant phenotype" Am. J. Pathol.144. 776-786 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Sugihara, et al.: "The promotion of invasion through the basement membrane of cervical carcinoma cells by fibronectin as a chemoattractant" Cancer, et al.167-173 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Sonoda, et al.: "Establishment of a new human uterine cervical adenocarcinoma cell line, SiSo, and its reactivity to anti-cancer reagents." Int. J. Oncology. 6. 1099-1104 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Saito et al.: "Antiproliferativelymphokine production by human peripheral blood lymphocytes and lymph node lymphocytes detected by a modified double layr soft agarose clonogenic assay." Lymphokine and Cytokine Research. 13. 55-62 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Kobayashi et al.: "Variant sublines of early-stage human melanomas selected for tumorigenicity in nude mice express a multicytokine-resistantphenotype." Am.J.Pathol.144. 776-786 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Sugihara et al.: "The promotion of invasion through the basement membrane of cervical carcinoma cells by fibronectin as a chemoattractant." Cancer.Lett.79. 167-173 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Sonoda et al.: "Establishment of a new human uterine cervical adenocarcinoma cell line, SiSo, and its reactivity to anti-cancer reagents." Int.J.Oncology. 6. 1099-1104 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Saito, et al.: "Antiproliferative lymphokine production by human peripheral blood lymphocytes and lymph node lymphocytes detected by a modified double layer soft agarose clonogenic assay" Lymphokine and Cytokine Research. 13. 55-62 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] H. Kobayashi, et al.: "Variant sublines of early-stage human melanomas selected for tumori genicity in nude mice express a multicytokine-resistant phenotype" Am. J. Pathol.144. 776-786 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Sugihara, et al.: "The promotion of invasion through the basement membrane of cervical carcinoma cells by fibronectin as a chemoattractant" Cancer. Lett.79. 167-173 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Sonoda, et al.: "Establishment of a new human uterine cervical adenocarcinoma cell line, SiSo, and its reactivity to anti-cancer reagents." Int. J. Oncology. 6. 1099-1104 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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