• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Electrophysiological study on GnRH secretion in GnRH cells

Research Project

Project/Area Number 06671672
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionYokohama City University School of Medicine

Principal Investigator

UEMURA Tsuguo  Yokohama City University School of Medicine, Department of Obstetrics and Gynecology Associate professor, 医学部, 助教授 (40046040)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Hajime  Yokohama City University School of Medicine, Department of Obstetrics and Gyneco, 医学部, 助手 (30254212)
SHIRASU Kazuhiro  Yokohama City University School of Medicine, Department of Obstetrics and Gyneco, 医学部, 講師 (60171047)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
KeywordsGnRH neuron / membrane potential / Ca^<2+> mobilization / GnRH / noradrenaline / GnRH律動的分泌 / patch clamp / multiple unit activity / Ca イオン / multiple unit activiry
Research Abstract

Noradrenaline (NA) is one of the most important neurotransmitters involved in the regulation of gonadotropin-releasing hormone (GnRH) secretion. In this study, the effects of NA on GnRH secretion, intracellular Ca^<2+> concentrations ([Ca^<2+>]i), and membrane potentials were investigated in immortalized hypothalamic neurons (GT1-7) to determine the direct effects of NA on GnRH cells.
Cells were perfused in a plastic minicolumn, and GnRH concentrations of the effluents were measured. NA increased the release of GnRH in a dose-dependent manner. Cells were loaded with 4 muM Fura 2-AM,and the ratio of the intensities of fluorescent emission at 510 nm with excitation at 340 and 380 nm was calculated at 100-ms intervals. NA increased the [Ca2+]i responses of single GnRH cells dose-dependently. The NA-induced [Ca^<2+>]i increase was attenuated in the absence of extracellular calcium and was blocked by the b-adrenergic antagonist propranolol, but not by the a-adrenergic antagonist phentolamine. The cell membrane potential was recorded with a whole-cell patch clamp amplifier with glass-electrodes. NA induced membrane depolarization under current-clamp conditions. The depolarization was also inhibited by propranolol, but not by phentolamine.
The results show that NA directly affects the membrane potential of GT1-7 cells via b-adrenergic receptors and induces Ca^<2+> mobilization ; these effects stimulate GnRH secretion.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Uemura T,Namiki T,et al.: "Direct effects of gonadotropin-releasing hormone on the ovary in rats and humans." Horm Res. 41(Suppl.1). 7-13 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uemura T,Mohri J,et al.: "Effect of gonadotropin-releasing hormone agonist on the bone mineral density of patients with endometriosis." Fertil Steril. 62(2). 246-250 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yang B,Uemura T,Minaguchi H.: "Effects of a gonadotropin releasing hormone agonist on oocyte maturation,fertilization,and embryonal development in mice." J Assisted Reproduction and Genetics. 12. 728-732 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uemura T,Hashimoto S: "Abnormal sexual differentiation." Asian Medical J. 39. 657-663 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Minaguchi H,Uemura T,Shirasu K,et al.: "Effects of estriol on bone loss in postmenopausal Japanese women:a multicenter prospective open study." J Obstet Gynaecol Res. 22. 259-265 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uemura T,Nishimura J,et al.: "Effects of noradrenalin on GnRH-secreting immortalized hypothalamic(GT1-7)neurons." Endocrine Journal. 44. 73-78 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uemura T,Yoshimura Y,et al.: "Prospectives on Assisted Reproduction" Ares-Serono Symposia, 749 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 植村次雄、松山明美: "図説産婦人科 VIEW-13、「生殖内分泌・不妊」生殖と神経内分泌-生殖内分泌の病態より深い理解をめざして-" メジカルビュー社, 187 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uemura T,Nishimura J,Yamaguchi H,Kimura F,Minaguchi H: "Electrophysiological effects of noradrenaline on GnRH neurons" Acta Obstetrica et Gynaecologica Japonica. 49 Supplement. S-499 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uemura T,Nishimura J,Yamaguchi H,Hiruma H,Kimura F,Minaguchi H: "Effects of noradrenalin on GnRH-secreting immortalized hypothalamic (GT1-7) neurons." Endocrine Journal. 44. 73-78 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uemura T,Hashimoto S.: "Abnormal sexual differentiation." Asian Medical J. 39. 657-663 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Minaguchi H,Uemura T,Shirasu K,et al.: "Effects of estriol on bone loss in postmenopausal Japanese women : a multicenter prospective open study" J Obstet Gynaecol Res. 22. 259-265 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 植村次雄: "GnRHの基礎と臨床" 日産婦会誌. 48. 672-677 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 植村次雄、石川雅彦、近藤芳仁、水口弘司: "子宮内膜症不妊に対するGnRHaの治療効果について" エンドメトリオーシス研究会誌. 17. 36-41 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 沼崎令子、植村次雄、山口肇、水口弘司、他: "子宮内膜症におけるCA125の診断の有用性についての検討" 日不妊会誌. 41. 359-364 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 何徳欽、植村次雄、遠藤方哉、水口弘司、他: "Interleukin-6(IL-6)のマウス2細胞胚および精子に及ぼす影響について" 日受精着床会誌. 149-152 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 植村次雄: "更年期[7]、骨粗鬆症(1)、症状と診断、女性のヘルスケア、ラジオたんぱ放送内容集(平成6年11月〜平成7年2月放送分)" 帝国臓器, 25-28 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 植村次雄: "内分泌トピックス[2]、GnRHおよびGnRHアナグロ、女性のヘルスケア、(19)性差・内分泌トピックス、ラジオたんぱ放送内容集(平成7年5月〜7月放送分)" 帝国臓器, 31-34 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yang B, Uemura T, Minaguchi H.: "Effects of a gonadotropin releasing hormone agonist on oocyte maturation,fertilization, and embryonal development in mice." J Assisted Reproduction and Genetics. 12. 728-732 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 董曦、植村次雄、八ツ橋良三、堀裕雅、水口弘司.: "マウス2細胞胚の凍結法についての検討-特に、凍結保護剤、非透過性耐凍剤について" 日不妊会誌. 40. 176-180 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 植村次雄、遠藤方哉、水口弘司: "腹腔鏡下手術、最近の進歩と将来の展望-子宮外妊娠-" 日産婦内視鏡誌. 10. 15-18 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 遠藤方哉、植村次雄、町田稔文、橋本栄、山口肇、近藤芳仁.: "レゼクトスコープ下に手術後、妊娠に至った粘膜下筋腫の二例について" 日産婦内視鏡誌. 10. 76-77 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 植村次雄、近藤芳仁: "性交障害とその対応" 日産婦誌. 47. N-235-N-238 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 植村次雄: "子宮内膜症の治療と問題点" 産婦治療. 71. 176-180 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 植村次雄、松山明美: "図説産婦人科VIEW-13、「生殖内分泌・不妊」生殖と神経内分泌-生殖内分泌の病態より深い理解をめざして-" メジカルビュー社, 187 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 水口弘司、植村次雄: "GnRH、図説産婦人科VIEW-13、「生殖内分泌・不妊」生殖と神経内分泌-生殖内分泌の病態より深い理解をめざして-" メジカルビュー社, 187 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Uemura,T.et al.: "Direct effects of gonadotropin-releasing hormone on the ovary in rats and humans" Horm Res. 41(Suppl.1).7-13. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Uemura,T.et al.: "Effect of gonadotropin-releasing hormone agonist on the bone mineral density of patients with endometriosis." Fertil Steril. 62. 246-250. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Uemura,T.et al.: "A classification of women with hypothalamic progestagen-negative amenorrhea" International Journal of Gynecology & Obstetrics. 46(Suppl 1).157.- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 植村次雄,段泰行.: "GnRH analogの婦人科腫瘍への応用." 産と婦. 61. 347-352 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 植村次雄.: "GnRH誘導体の排卵誘発、IVFへの応用." 産と婦. 61. 1143-1448 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 植村次雄,遠藤方哉.: "GnRHの卵巣への直接作用." Hormone Frontier in Gynecology. 1. 53-60. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Uemura,T.et al.: "Frontiers in Endocrinology Vol4;Prospectives on Assisted Reproduction." Rome:Ares-Serono Symposia,749 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 植村次雄(共著): "産婦人科学書1、生殖医学" 金原出版, 862 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi