| Project/Area Number |
06671674
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | OSAKA CITY UNIVERSITY |
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Principal Investigator |
UMESAKI Naohiko OSAKA CITY UNIVERSITY MEDICAL SCHOOL ASSOCIATE PROFESSOR, 医学部, 助教授 (20106339)
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| Co-Investigator(Kenkyū-buntansha) |
FUJINO Yuuji OSAKA CITY UNIVERSITY MEDICAL SCHOOL,LECTURER, 医学部, 講師 (00201749)
OGITA Sachio OSAKA CITY UNIVERSITY MEDICAL SCHOOL,PROFESSOR, 医学部, 教授 (00047086)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | EMBRYONIC DEVELOPMENT / ENDOMERTRIUM / ENDOMETRIAL STROMAL CELL / ENDOMETRIOSIS / PERITONEAL FLUID / Endometrial stromal cell / 子宮内膜間質細胞 / 受精卵成熟促進因子 / 子宮内膜症 / 体外受精・胚移植 |
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| Research Abstract |
Embroys implant at endometrium after development in the tube and intra-uterine cavity. So endometrium may play a role of enhancement of embryonic development. In this study, we evaluated the action of endometrium on early embryonic development by using co-culture system with endometrial stromal cells (ESCs) and mice embryos. ESCs were obtained from patients with leiomyoma (control) or endometriosis (EM). ESCs from control increased the embryonic development in comparison with EMS.Peritoneal fluid (PF) from EM suppressed the embryonic development in the co-culture system. Next the influence of serum from EM patients on embryonic development was evaluated. This suppressed the embryonic development. Further, culture of embryo in the culture medium with erythrocytes enhanced the embryonic development. In this studies, co-culture with SMC from control or erytrocytes in culture medium enhanced the embryonic developments and co-culture with stromal cells from EMS and PF or serum from EM suppresed th embryonic development. Now we are studying about enhancing factor from endometrium and suppresing factor PF or serum from EM on embryonic divelopment.
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