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Modulation of immune system by transforming growth factor-beta in human milk

Research Project

Project/Area Number 06671675
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionNara Medical University

Principal Investigator

ISHIZAKA Shigeaki  Nara Med.Univ.Parasitol.Professor, 医学部, 教授 (90159715)

Co-Investigator(Kenkyū-buntansha) SAITO Shigeru  Nara Med.Univ.Obstetrics & Gynecol.Lecture, 医学部, 講師 (30175351)
荒木 恒治  奈良県立医科大学, 医学部, 教授 (30084867)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsTGF-beta / Liver cells / Antibody production / Mouse / Human milk / PCR / ELISPOT / IL-10 / 経口投与 / 羊赤血球抗原
Research Abstract

Human milk contains large amounts of transforming growth factor-beta (TGF-beta), which exerts powerful suppressive effects on many immune functions in vitro. Oral administration of human milk and TGF-beta in mice strongly inhibited antisheep red blood cell (SRBC) responses obtained following oral immunization with SRBC,but enhanced the responses elicited by intraperitoneal, intrabronchial or intravenous immunization with SRBC.Anti-SRBC antibody responses stimulated by intraperitoneal or intravenous immunization with SRBC failed to augment when TGF-beta -depleted human milk was given orally. Continuous intrahepatic administration of TGF-beta augmented IgM,IgG and IgA antibody production of mouse spleen cells. Furthermore, the supernatant from normal liver cell (Clone 1469) culture enhanced polyclonal antibody production in mouse spleen cells but not in human peripheral blood lymphocytes. TGF-beta led to augmentation of IL-10 secretion from Clone 1469 cells. Therefore, IL-10 secreted by TGF-beta-stimulated hepatocytes may play an essential role in enhancement of antibody production.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ishizaka,S. et al.: "Antibody production system modulated by oral administration of human milk and TGF-β." Cell.Immunol.159. 77-84 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 吉川正英,他: "TGF-β肝内持続投与の免疫グロブリン産生に及ぼす影響" 肝臓. 35. 395-396 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 石坂重昭: "母乳中のTGF-βは経口抗原に対する抗体産生を抑え非経口抗原に対する抗体産生を増幅する。" 臨床免疫. 27. 471-476 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshikawa,M.et al.: "Effects of continuous intrahepatic administration of transforming growth factor-beta on immunoglobulin production" Int.Hepatol.Commun.3. 116-120 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishizaka,S.et al.: "IL-10 production in mouse hepatocytes augmented by TGF-β" Cytokine. (予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishizaka, S., Kimot, M., Tsujii, T.and Saito, S: "Antibody prodution system modulated by oral administeation of human milk and TGF-beta" Cell.Immunol. 159. 77-84 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishizaki, S: "TGF-beta in human milk suppressed the antibody prodution stimulated by oral immunization, but enhanced the antibody responses against parenteral antigens. (Japanese)" Clin.Immunol. 27. 471-476 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshikawa, M., Matsui, Y., Nakatani, T., Yamao, J., Kuriyama, S., Ishizaka, S., Fukui, H., and Tsujii, T: "Effects of continuous intrahepatic administration of transforming growth factor-beta on immunoglobulin production" Int.Hepatol.Commun. 3. 116-120 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshikawa,M.,et al: "Effects of continuous intrahepatic administration of transforming growth factor-beta on immunoglobulin production." Int.Hepatol.Commun.3. 116-120 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 石坂重昭: "母乳中のTGF-βは経口抗原に対する抗体産生を抑え非経口抗原に対する抗体産生を増幅する" 臨床免疫. 27. 471-476 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 木本誠: "TGF-β刺激による上皮細胞からの免疫活性因子" 日本免疫学会総会・学術集会記録. 25. 143 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 石坂重昭: "TGF-β様作用を示す抗生剤ホスホマイシンの免疫薬理活性" 日本免疫学会総会・学術集会記録. 25. 259 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishizaka,s.et al.: "Antibody production system modulated by oral administration of human milk and TGF-β" Cellular Immunology. 159. 77-84 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 石坂重昭: "母乳中のTGF-βは経口抗原に対する抗体産生を抑え非経口抗原に対する抗体産生を増幅する。" 臨床免疫. (印刷中).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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