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THE ROLE OF CYTOKINES FOR MAINTENANCE OF PREGNANCY

Research Project

Project/Area Number 06671676
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionNARA MEDICAL UNIVERSITY

Principal Investigator

SAITO Shigeru  NARA MEDICAL UNIVERSITY,OBSTET & GYNECOL., LECTURER, 医学部, 講師 (30175351)

Co-Investigator(Kenkyū-buntansha) 榎本 匡浩  奈良県立医科大学, 医学部, 助手 (60254504)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsplacenta / decidua / cytokine / NK cell / implantation / IL-8 / M-CSF / SCF / HGF / c-kit
Research Abstract

Varieties of cytokine receptors are present on trophoblast infiltraing into the decidua, and many cytokines are produced in the decidua. In the present study, we examined physiological effects of these cytokines on the trophoblast in vitro, and found that growth of the trophoblast was accelerated with M-CSF,SCF,HGF and EGF.Interestingly, all of the receptors for these cytokines contain a tyrosine kinase domain in themselves. This finding indicates the importance of tyrosine kinase in the growth of the trophoblast. On the other hand, it was concluded that EGF and M-CSF promote the differentiation, SGF suppresses thedifferentiation on the contraray, and HGF dose not affect the differentiation atall. In other words, it was clarified that tyrosine kinase is used as a common pathway in acceleration of growth of the trophoblast, but different complicated signal transduction pathways for induction of differentiation exist.
In the present study, we also clarified production of varieties of cyto … More kinesby CD16-CD56 ^<bright>NK cells that markedly proloferated in the decidua in the early stage of pregnancy. Among them, M-CSF was showm tocontribute to the growth and differentiation of the prophoblast. Since angiogenesis effect of IL-8 has been reported, its participation in angiogenesis at the time of implantation can be expected. When GM-CSF was administrered to abortive mice, CBA*DBA_2, the abortion rate decreased. Hence, GM-CSF secreted by decicual CD16^- CD56^<bright> NK cells appear to play some role in implantation. Since IFN and TNF alpha promotethe expression of MHC Class I molecule and may have been protected from the attack by NK cells. TGF beta produced by decidual NK cells and trophoblast suppressed production of IL-2 decidual T-cells, and appeared to protect the prophoblast from the attack by NK cells, and as a result TGF beta contribute to maintanence of pregnancy. In summary, cytokines appear to cotribute to the maintanence of pregnancy by controlling the immune system and promoting the function of the trophoblast at the implantation site. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 斎藤 滋 他: "妊娠初期脱落膜リンパ球についての検討" 産婦人科の進歩. 48(掲載予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Saito,S.et al: "Interleukin 4(IL-4)blocks the IL-2 induced in NK activity and DNA synthesis of decidual CD16^-CD56^<bright>NK cells by inhibiting expression of the IL-2 receptorα,β and_γ" Cell Immunol.(IN PRESS). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishii,E.et al: "Produciton and expression of granulocyte and macrophage colony-stimulating factors in newborns : Their roles in leukocytosis at birth" Acta Haematologica. 94. 23-31 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Saito,S.et al: "Hepatocyte growth factor promotes the growth of cytotrophoblasts by paracrine mechanism" J.Biochem.117. 671-676 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayakawa,S.et al: "Expression of recombinase-activating genes (RAG-1 and 2) in human decidual mononuclear cells" J.Immunol.153. 4934-4939 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Saito,S.et al: "Localization of stem cell factor (SCF) and c-Kit mRNA in human placental tissue and biological effects of SCF on DNA synthesis in primary cultured cytotrophoblast" Biochem.Biophys.Res.Commun.205. 1762-1769 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Saito S.et al: "Interleukin 4 (IL-4) blocks the IL-2 induced increase in NK activity and DNA synthesis of decidual CD16^-CD56^<bright> cells by inhibiting expression of the IL-2 receptor alpha, beta and gamma" Cell Immunol. Vol.170. 71-77 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishii E.et al: "Peoduction and expression of granulocyte and macrophage colonystimulating factors in newborns : Their roles in leukocytosis at birth" Acta Haematologica. Vol.94. 23-31 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Saito S.et al: "Hepatocyte growth factor promotes the growth of cytotrophoblasts by paracrine mechanism" J Biochem. Vol.117. 671-676 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayakawa S.et al: "Expression of recombinase-activating genes (RAG-1 and 2) in human decidual mononuclear cells" J.Immunol. Vol.153. 4934-4939 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Saito S.et al: "Localization of stem cell factor (SCF) and c-kit mRNA in human placental tissue and biological effects of SCF on DNA synthesis in primary cultured cytotrophoblast" Biochem.Biophys.Res.Commun. Vol.205. 1762-1769 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 斎藤滋,他: "妊娠初期脱落膜リンパ球についての検討" 産婦人科の進歩. 48(掲載予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Saito,S.,et al: "Interleukin 4(IL-4)blocks the IL-2 induced in NK activity and DNA synthesis of decidual CD16^-CD56^<bright>NK cells by inhibiting expression of the IL-2 receptorα,β andγ" Cell Immunol.INPRESS. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishii,E.,et al: "Produciton and expression of granulocyte and macrophage colony-stimulating factors in newborns:Their roles in leukocytosis at birth" Acta Haematologica. 94. 23-31 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Saito,S.,et al: "Hepatocyte growth factor promotes the growth of cytotrophoblasts by paracrine mechanism" J.Immunol.117. 671-676 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hayakawa,S.,et al: "Expression of rocombinase-activating genes(RAG-1 and 2)in human decidual mononuclear cells" J.Immunol.153. 4934-4939 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Saito,S.,et al: "Localization of stem cell factor(SCF)and c-kit mRNA inhuman placental tissue and biological effects of SCF on DNA synthesis in primary cultured cvtotrophoblast" Biochem.Biophys.Res.Commun.205. 1762-1769 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Saito S et al: "Macrophage colony-stimulating factor induces the growth and diifferentiation of normal paregnancy human cytotrophoblast cells and hydatidityon moles" Jpn.J.Cancer.Res. 85. 245-252 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Saito S et al: "Expression of granulcyte colony-stimulating factor and its receptor at the fetomaternal interface in murine and human pregnancy" Growth Factors. 10. 135-143 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Saito S et al: "Interleukin-8 production by CD16^-CD56^<bright> natural killer cells in the human early pregnancy decidua" Biochem Biophys Res Commun. 200. 378-383 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Saito S et al: "Localization of stem cell factor(SCF) and c-kit mRNA in human placental tissue and biological effects of SCF on DNA synthesis in primary cultured cytotrophoblasts" Biochem Biophys Res Commun. 205. 1762-1769 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Saito S et al: "Detection and localization of interleukin-8 mRNA and protein in human placenta and decidual tissues" J.Reprod.Immunol. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Saito S et al: "Hepatocyte growth factor promotes the growth of cytotrophoblasts by the paracrine mechanism" J.Biochem. 117(in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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