A novel oncodevelopmental protein

• Project/Area Number: 06671681
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Kitasato University
• Principal Investigator: NAKAI Mitsuo Kitasato Univ., Sch.of Medicine, Professor, 医学部, 教授 (50050399)
• Co-Investigator(Kenkyū-buntansha): KENMOCHI Minoru Kitasato Univ., Sch.of Medicine, Research Assoc., 医学部, 助手 (10205289) ; IINO Joji Kitasato Univ., Sch.of Medicine, Research Assoc., 医学部, 助手 (30176052) ; ISHIKAWA Masakazu Kitasato Univ., Sch.of Medicine, Research Assoc., 医学部, 助手 (40184494) ; KAWAUCHI Hirohito Kitasato Univ., Sch.of Medicine, Asst.Professor, 医学部, 講師 (90152917) ; YAMAURA Noboru Kitasato Univ., Sch.of Nursing, Assoc.Professor, 看護学部, 助教授 (60050621)
• Project Period (FY): 1994 – 1995
• Project Status: Completed (Fiscal Year 1995)
• Budget Amount: ¥2,000,000 (Direct Cost: ¥2,000,000); Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000); Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: Tumor-associated protein / Oncodevelopmental protein / Malignant ascitic fluid / Isolation / Amino acid sequence / Ca-binding protein MRP8 / Tumor marker / 腹水抗原 / 純化 / 妊娠関連蛋白

Research Abstract

1. Isolation of an Ascitic Oncodevelopmental Protein Exhibiting High Sequence Homology with Calcium-Binding Protein MRP8
A human oncodevelopmental protein (ODP) with a molecular size of 28 kDa was isolated, employing malignant ascitic fluid of an advanced mixed mesodermal tumor of the uterus, by anti-ODP coupled affi-Gel 10 column chromatography, followed by preparative PAGE.The final preparation obtained with this procedure gave a single band exhibiting reactivity with an anti-ODP antibody on analytical sodium dodecyl sulfate-PAGE.The sequence of the first 20 N-terminal amino acids of this protein is identical with those of both the cystic fibrosis protein and Ca-binding protein MRP8 except at position 17, for which no result was obtained. Sequence analysis suggested that tha protein shows 90% sequence homonlogy with both these proteins.
2. Immunolocalization of 91 kDa Ascitic Protein in the Human Placenta
A 91 kDa protein, sharing antigenicity with a pregnancy-associated antigen, was is olated from the ascitic fluid of a patient suffered from the gynaecological malignant neoplasms. It was revealed in the human placenta by immunocytochemical techniquies, and found to be localized in the stroma, including a fibrous network, fibroblasts and the Hofbauer cells of the terminal and stem villi in the first trimester. Immunoreactive staining was also found in macrophage-like cells, presumable the precursor of the Hofbauer cell, in the stem villi of placenta. It is assumed that macrophage-like cells containing the protein would be originated from the extraembryonic mesoderm or blood vessels, and then migrated to the stroma of terminal villi. During the second trimester, the protein was observed in numerous vesicles among the syncytial trophoblasts. The density of these immunoreactive vesicles and the cytoplasm of Hofbauer cells was rapidly increased with gestational stages, whereas it was gradually reduced towards the late-second trimester. The highest labellings occurred at around 17 weeks or later. On the other hand, at term, the protein was mainly observed in numerous villous blood vessels including endothelial cells.

Research Products

• [Publications] Kami,K,Sato,N,Morita,T,Ishiwata,I,Ishikawa,M & Nakai,M: "Immunolocalization of 91 kDa Ascitic Protein in the Human Placenta." Okajimas Folia Anat Jpn. 72. 227-234 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakai,M.,Ishikawa,M,Hamada,Y.and Sugano,: "Isolation of an Ascitic Oncodevelopmental Protein Exhibiting High Sequence Homology with Calcium-Binding Protein MRP8." Biol Chem Hoppe-Seyler. 375. 789-792 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kami, K., Sato, N., Morita, T., Ishiwata, I., Ishikawa, M.and Nakai, M.: "Immunolocalization of 91 kDa Ascitic Protein in the Human Placenta" Okajimas Folia Anat Jpn. 72 (4). 227-234 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakai, M., Ishikawa, M., Hamada, Y.and Sugano, S: "Isolation of an Ascitic Oncodevelopmental Protein Exhibiting High Sequence Homology with Calcium-Binding Protein MRP8" Biol Chem Hoppe-Seyler. 375. 789-792 (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kami, K, Sato, N, Morita, T, Isiwata, I, Ishikawa, M, Nakai, M.: "Immunsocaligation of 91 kDa Ascitic Protein in the Human Placenta" Okajimas Folia Anat Jpn. 72. 227-234 (1995)
• [Publications] Mitsuo Nakai: "Isolation of an ascitic oncodeuelopmental protein exhibiting high sequonce homslogy with Calciuen-Binding Protein MRP8" Biological Chemistry Hoppe-Seyler. 375. 789-792 (1994)