Study on the selection of normal sperm and the method of fusion of sperm and oocytes for micro-feritlization.
| Project/Area Number |
06671682
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kyorin University |
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Principal Investigator |
JINNO Masao Kyorin University, School of Medicine, Department of Obstetrics and Gynecology, Assistant Professor, 医学部, 講師 (00162826)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | sperm / male infertility / fertilization / micro-fertilization / in vitro fertilization / 顕微受精 / 体外受精 |
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| Research Abstract |
The swim-shift (SS) methoda novel method of sperm selection, was invented. It retrieved more motile sperm than the conventional swim-up (SU) method. The PSA-column method retrieved morphologically normal sperm selectively. When SS method and PSA-column method were combined, a sperm concentration and %norma morphology were higher and %mortility was similar, compared with the SU method alone. Several procedures of subzonal insemination of sperm (SUZI) were modified to give a minimal %damage (%D) and maximal %fertilization (%F) of hamster oocytes with human sperm without use of sucrose for expansion of subzonal space, becaouse sucrose toxicity to oocytes was suggested. Pretreatment of sperm with 5 muM calcium ionphore improved %F of zona-free hamster oocytes. When the improved procedures of SUZI was applied for treatments of severe male infertility, %F and %embryo cleavage were improved significantly but %pregnancy was not, compared with the conventional procedures of SUZI.When sperm concentration of sperm droplet was diluted by 100 times to give 100,000 per ml, %F of hamster oocytes with human sperm by intracytoplasmic sperm injection (ICSI) was significantly increased. It was suggested that incision of sperm midpiece just before ICSI increased %F.A use of Piezo-manipulator decreased %D and increased %F significantly. When the improved procedures of ICSI with dilution of sperm concentration, incision of sperm midpiece, and use of Piezo-manipulator was applied for treatments of severe male infertility, numbers of fertilized oocytes and cleaved embryos and %pregnancy per cycle were significantly increased compared with conventional procedures of ICSI,resulting in 20% of ongoing pregnancy rate per cycle.
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Report
(3 results)
Research Products
(3 results)
