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Gene alterations of glycosyltransferase during the course of malignant tranformation in uterine endometrium

Research Project

Project/Area Number 06671685
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKeio University

Principal Investigator

AOKI Daisuke  School of Medicine, Keio University, Assistant Professor, 医学部, 講師 (30167788)

Co-Investigator(Kenkyū-buntansha) UDAGAWA Yasuhiro  School of Medicine, Keio University, Assistant Professor, 医学部, 講師 (80118918)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
KeywordsEndometrial cancer / Carbohydrate antigen / Fucosyltransferase / PCR-RFLP / Le gene / Se gene / モノクローナル抗体MSN-1 / 糖転移酵素 / フコース転移酵素-III / モノクローナル抗体 MSN-1 / ルイス型関連抗原
Research Abstract

Monoclonal antibody MSN-1 was established against an endometrial cancer cell line and proven to recognize at least Lewis b carbohydrate antigen. Immunohistochemical staining with this antibody resulted in a positive reaction in more than 85% of the endometrial adenocarcinoma cases examined but in few cases with normal endometria, indicating that MSN-1-reactive antigen is able to be a good histochemical marker for endometrial cancers. Expression of the Lewis histo-blood group antigen is determined by the Lewis type alpha (1,3/1,4) fucosyltransferase enconded by the FucT-III gene (Le gene). Since Lewis-negative individuals are known to be homozygous for an allele with missense mutations of this gene (le/le) which cause inactivation of the enzyme activity, we investigated whether or not phenotypic expression of the MSN-1-reactive antigen in endometrial adenocarcinoma specimens was affected by the genotypes of the FucT-III gene in patients with adenocarcinoma. To determine the genotypesof the FucT-III gene, i.e., to detect the mutations, we employed the polymerase chain reaction (PCR) -restriction fragment length polymorphism method by using genomic DNAs extracted from the patients'peripheral blood leucocytes as PCR templates. Out of 59 cases of endometrial adenocarcinoma, four cases with the Lewis-negative genotype (le/le) were identified and examined by the ABC immunohistochemical technique for the presence of the MSN-1-reactive antigen on their cancer cells. The conventional hemagglutination reaction also revealed all four of these cases to have the Lewis-negative blood type. However, their cancer tissues reacted positively with MSN-1. These results suggest that MSN-1 recognizes a fucosylated carbohydrate that is constructed by fucosyltransferases other than FucT-III.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Aoki,D: "Phenotypic alterations of a carbohydrate antigen,blood group A type 3 chain,in neoplastic transformation of uterine cervix" Med.Electron Microscopy. 27. 312-314 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 青木大輔: "細胞診の見方と考え方〔27〕培養細胞を免疫源として作製したモノクローナル抗体による免疫組織細胞化学的解析" 臨床と病理. 12. 1235-1241 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoki,D: "Phenotypic alteration of carbohydrate antigens in gynecological malignancy" Acta Histochem.Cytochem.28. 197-201 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukuda,M.N.: "Trophinin and tastin;a novel cell adhesion molecule complex with potential involvement in embryo implantation" Genes and Development. 9. 1199-1210 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akimoto,Y.: "Immunocytochemical localization of the protein reactive to human β 1,4-galactosyltransferase antibodies during chick embryonie skin differentiation" The Anatomical Record. 234. 109-119 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 斉藤英子: "新たな腫瘍マーカーを用いた卵巣がんスクリーニングの試み" 産業衛生学雑誌. 37. S269 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 斉藤英子: "Western blottingを中心とした卵巣癌細胞のGT/GAT産生能の検索" 腫瘍マーカー研究会誌. 11. 175-177 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 斉藤美和: "CA602・CA546及び超音波断層法の組み合わせによる卵巣腫瘍の良・悪性の鑑別における診断能" 腫瘍マーカー研究会誌. 11. 39-41 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 青木大輔: "子宮体癌の手術療法の選択" 癌と化学療法. 22. 1163-1168 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoki,D.: "β 1,4-galactosyltransferase expressed in ovarian cancer cells" Acta Histochem.Cytochem.29. 232-233 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 宇田川康博: "糖鎖系腫瘍マーカー-その他の糖鎖関連腫瘍マーカー-図説産婦人科VIEW「腫瘍編」(加藤 紘,野澤志朗編)" メジカルビュー社, 10 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.Aoki, N.Susumu, H.Kawakami, H.Hirano, and S.Nozawa: "Phenotypic alteration of carbohydrate antigens in gynecological malignancy" Acta Histochem.Cytochem.28. 197-201 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.N.Fukuda, T.Sato, J.Nakayama, G.Klier, M.Mikami, D.Aoki, and S.Nozawa: "Trophinin and tastin ; a novel cell adhesion molecule complex with potential involvement in embryo implantation" Genes and Development.9. 1199-1210 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Akimoto, A.Obinata, H.Endo, K.Furukawa, D.Aoki, S.Nozawa, and H.Hirano: "Immunocytochemical localization of the protein reactive to human beta1,4-galactosyltransferase antibodies during chick embryonic skin differentiation" The Anatomical Record.234. 109-119 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.Aoki, E.Saitoh, N.Susumu, H.Kawakami, T.Uejima, Y.Udagawa, H.Hirano, and Shiro Nozawa: "beta1,4-galactosyltransferase expressed in ovarian cancer cells" Acta Histochem.Cytochem.29. 232-233 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoki,D: "β1,4-galactosyltransferase expressed in ovarian cancer cells" Acta Histochem.Cytochem.29. 232-233 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Aoki, D.: "Phenotypic alteration of carbohydrate antigens in gynecological malignancy" Acta Histochem. Cytochem.28 (2). 197-201 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Aoki, D.: "Phenotypic alterations of a carbohydrate antigen, blood group A type 3 chain, in neoplastic transformation of uterine cervix" J. Clin. Electron Microscopy. 27 (3-4). 312-314 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Aoki, D.: "Successful heterotransplantation of human endometrium in SCID mice" Obstet. Gynecol.83. 220-228 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Susumu, N.: "Subcellular locailzation of blood group Leb carbohydrate antigen (MSN-1-reactive antigen) in endometrial cancer cells" Cancer Res.53. 3643-3648 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Aoki,D.: "Phenotypic alteration of carbohydrate antigens in gynecological malignancy" Acta Histochem.Cytochem.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Aoki,D.: "Phenotypic alterations of a carbohydrate antigen,blood group A type 3 chain,in neoplastic transformation of uterine cervix" J.Clin.Electron Microscopy. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Aoki,D.: "Successful heterotransplantation of human endometrium in SCID mice" Obstet.Gynecol.83. 220-228 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Susumu,N.: "Subcellular localization of blood group Leb carbohydrate antigen (MSN-1-reactive antigen) in endometrial cancer cells" Cancer Res.53. 3643-3648 (1993)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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