| Project/Area Number |
06671687
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
SUEOKA Kou Keio Univ., School of Medicine, assistant, 医学部, 助手 (90162833)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIBA Tsuyoshi Keio Univ., School of Medicine, assistant, 医学部, 助手 (60245553)
ASADA Hironori Keio Univ., School of Medicine, assistant, 医学部, 助手 (60231883)
KUJI Naoki Keio Univ., School of Medicine, assistant, 医学部, 助手 (80169987)
KOBAYASHI Toshifumi Keio Univ., School of Medicine, associate professor, 医学部, 助教授 (30051460)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | EPF / Rosette inhibition test / SDS-PAGE / Glycoprotein / Western blot / RP-HPLC / Thioredoxin / Chaperonin 10 |
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| Research Abstract |
Human early pregnancy factor (EPF) has been elucidated in the biochemical characteristics, chemical structure and the bioactivity specially for the essential role of EPF in the mechanisms of establishment of pregnancy.
The purified property from the source of human urine from pregnant women has been determined amino acid sequences from N-terminal of its peptide. Our purified substance with EPF bioactivity had 28KD of molecular weight and acidic peptide.
The other purified properties of EPF are from the extract of human placenta had been recently reported by Clarke et al., which had the identified sequences with Thioredoxin (Adult type T cell leukemia factor ; ADF). And Chaperonin 10 homology peptide purified from human serum by Morton et al.which have 21KD of molecular weight and acidic peptide similar with our isolated peptide. In order to identify these rwo EPF properties, the polyclonal antibodies has been made by immunizind synthesized peptides with parts of EPF chemical structure to the rabbit. The response for biological activity (rosette inhibition activity) and serological responses (Dot blot, ELISA), with this antibody, and biochemical determination have demonstrated that these three EPF-bioactive properties were suggested to consists of completely different biochemical structures.
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