• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The determination of amino acid sequence of human early pregnancy factor and the research on its bioactivity

Research Project

Project/Area Number 06671687
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKeio University

Principal Investigator

SUEOKA Kou  Keio Univ., School of Medicine, assistant, 医学部, 助手 (90162833)

Co-Investigator(Kenkyū-buntansha) HASHIBA Tsuyoshi  Keio Univ., School of Medicine, assistant, 医学部, 助手 (60245553)
ASADA Hironori  Keio Univ., School of Medicine, assistant, 医学部, 助手 (60231883)
KUJI Naoki  Keio Univ., School of Medicine, assistant, 医学部, 助手 (80169987)
KOBAYASHI Toshifumi  Keio Univ., School of Medicine, associate professor, 医学部, 助教授 (30051460)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsEPF / Rosette inhibition test / SDS-PAGE / Glycoprotein / Western blot / RP-HPLC / Thioredoxin / Chaperonin 10
Research Abstract

Human early pregnancy factor (EPF) has been elucidated in the biochemical characteristics, chemical structure and the bioactivity specially for the essential role of EPF in the mechanisms of establishment of pregnancy.
The purified property from the source of human urine from pregnant women has been determined amino acid sequences from N-terminal of its peptide. Our purified substance with EPF bioactivity had 28KD of molecular weight and acidic peptide.
The other purified properties of EPF are from the extract of human placenta had been recently reported by Clarke et al., which had the identified sequences with Thioredoxin (Adult type T cell leukemia factor ; ADF). And Chaperonin 10 homology peptide purified from human serum by Morton et al.which have 21KD of molecular weight and acidic peptide similar with our isolated peptide. In order to identify these rwo EPF properties, the polyclonal antibodies has been made by immunizind synthesized peptides with parts of EPF chemical structure to the rabbit. The response for biological activity (rosette inhibition activity) and serological responses (Dot blot, ELISA), with this antibody, and biochemical determination have demonstrated that these three EPF-bioactive properties were suggested to consists of completely different biochemical structures.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 久慈直昭: "新しいhCG検出試薬オ-ラテックhCGの臨床的検討" 産婦人科の実際. 43. 1865-1871 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 末岡浩: "高感度ヒト絨毛ゴナドトロピン測定EIAキット;(HCG)「SRL」の基礎的検討および早期妊娠診断への臨床応用" 医学と薬学. 33. 273-278 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 末岡浩: "受精および初期胚環境としての卵管内病態の検討-卵管鏡下卵管形成システムを用いて" 日本受精着床学会雑誌. 12. 85-88 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 黒島正子: "Minimal damage embryo biopsyの技術的検討" 日本受精着床学会雑誌. 13(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 橋場剛士: "着床前受精卵の性分析に関する研究-Capillary PCR法を用いた迅速な性分析法の開発-" 日本受精着床学会雑誌. 13. (1996(in press))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 末岡浩: "卵管-基礎から臨床まで-" 南山堂, 179-184 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 末岡浩: "卵管-基礎から臨床まで-" 南山堂, 207-214 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Sueoka, H.Asada, T.Hashiba, N.Kuji, T.Miyazaki, T.Kobayashi & S.Nozawa: "The falloposcopic findings of entire fallopian tube as a circumstance of fertilization and early development of embryos" journal of Fertilization and Implantation. 12 (1). 85-88 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Kuroshima, K.Sueoka, T.Hashiba, H.Asada, M.Horii, E.Saito, N.Kuji, T.Miyazaki, T.Kobayashi & S.Nozawa: "The technical evaluation of embryo biopsy procedures for minimal damage on embryo development." Journal of Fertilization and Implantation. 13 (1) (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Sueoka: "Fallopian tube.(Basic and Clinical Finding.)" Nanzando, Tokyo, March 30. 179-184 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Sueoka: "Early pregnancy factor. (Basic and Clinical Finding.)" Nanzando, Tokyo, March 30. 207-214 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 久慈直昭: "新しいhCG検出試薬オ-ラテックhCGの臨床的検討" 産婦人科の実際. 43. 1865-1871 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 末岡浩: "高感度ヒト絨毛ゴナドトロピン測定EIAキット;(HCG)「SRL」の基礎的検討および早期妊娠診断への臨床応用" 医学と薬学. 33. 273-278 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 末岡浩: "受精および初期胚環境としての卵管内病態の検討-卵管鏡下卵管形成システムを用いて" 日本受精着床学会雑誌. 12. 85-88 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 黒島正子: "Minimal damage embryo biopsyの技術的検討" 日本受精着床学会雑誌. 13 (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 橋場剛士: "着床前受精卵の性分析に関する研究-Capillary PCR法を用いた迅速な性分析法の開発-" 日本受精着床学会雑誌. 13 (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 末岡浩: "卵管-基礎から臨床まで-" 南山堂, 179-184 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 末岡浩: "卵管-基礎から臨床まで-" 南山堂, 207-214 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 末岡 浩: "受精および初期胚環境としての卵管内病態の検討-卵管鏡下卵管形成システムを用いて-" 日本受精着床学会雑誌. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 末岡 浩: "卵管-基礎から臨床まで-" 南山堂(in press), (1995)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi