| Project/Area Number |
06671690
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | TOKYO DENTAL COLLEGE |
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Principal Investigator |
KANEKO Satoru Tokyo Dental College, Lecturer, 歯学部, 講師 (40214457)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Acrosin / acrosome reaction / Concanavalin A / Cell affinity chromatography / Equilibrium sedimentation / 先体反応 / 卵膜融解 |
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| Research Abstract |
1. The acrosome reacted human sperm with progressive motility was purified by using cell affinity chromatography on Concanavalin A Sepharose.
2. The un-acrosome reasted sperm was separated by means of the continuous-step density gradient with an inner capiral, to avoid induction of the acrosome reaction, Percoll was made isotonic with Ca, NaHCO_3 free Hanks solution.
3. Hystochemical staining with FITC-Con A achieved to distinguish the acrosome reacted and un reacted sperm, the inner acrosome membrane exposed in consequence of the acrosome reaction had affinity against the agent.
4. Equilibrium sedimentation in Percoll revealed that the acrosome reacted sperm had lighter apparent density than those un-reacted.
5. Human sperm contained at least two arginine amidases. The above results, however, suggested it is difficult to monitor the acrosome reaction by activation of acrosin, which bound to the inner acrosome membrane.
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