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Physiological significance of IGFBP-1 phosphorylation on fetal growth

Research Project

Project/Area Number 06671692
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionTokyo Women's Medical College

Principal Investigator

IWASHITA Mitsutoshi  Tokyo Women's Medical College, Dept.of OB/GYN,Associate Professor, 医学部, 助教授 (30124936)

Co-Investigator(Kenkyū-buntansha) SAKAI Keiji  Tokyo Women's Medical College, Dept.of OB/GYN,Assistant, 医学部, 助手 (30260962)
KUDO Yoshiki  Tokyo Women's Medical College, Dept.of OB/GYN,Assistant Professor, 医学部, 講師 (80241082)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsGrowth factor / Fetus / Growth / IGF / IGF-binding protein / Phosphorylation / Metabolism / 胎児発育
Research Abstract

Insulin-like growth factor-I (IGF-I) is one of growth factors that circulates bound to specific, high affinity binding proteins (IGFBPs). One of IGFBPs, IGFBP-1 is known to be phosphorylated in biological fluid, however, the physiological significance of phosphorylation on this molecle is not elucidated. We investigated the effect of phosphorylation of IGFBP-1 on fetal growth in this study.
The isomers of phosphorylated IGFBP-1 in fetal rats and cord sera are separated by immunoblot and anion ion exchange chromatography in which one nonphosphorylated and four phosphorylated IGFBP-1 are detected. In pared blood samples from mid-term delivery, percentage of nonphosphorylated IGFBP-1 is higher in fetal blood compared to those in mother. Similarly, percentage of nonphosphorylated IGFBP-1 is elevated in AFD infants than in SFD infants from term delivery. Thus, the proportion of nonphosphorylated and phosphorylated isomers of IGFBP-1 varies corresponding to fetal growth.
The affinity of phosphorylated IGFBP-1 for IGF-I is 4-fold higher than those of nonphosphorylated IGFBP-1 when evaluated by binding study.
Nonphosphorylated IGFBP-1 enhances IGF-I-mediated ^3H-AIB uptake by cultured trophoblast cells while phosphorylated IGFBP-1 inhibits when cells are incubated with IGF-I under the presence of these isoforms of IGFBP-1.
Thus, phosphorylated and nonphosphorylated IGFBP-1 have differant biochemical property and modify IGF-I action differntly.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 岩下光利: "IGF-Iと胎児発育" 医学のあゆみ. 171. 611-614 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 岩下光利: "胎児発育と成長因子" ペリネイタルケア. 165. 81-86 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 岩下光利: "胎児発育とIGF" 内分泌・糖尿病科. 1. 459-468 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 岩下光利: "IGFと胎児発育" HORMONE FRONTIER. 2. 57-61 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 工藤美樹: "胎児発育・成熟とその異常" メジカルビュー社, 193 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 岩下光利: "生殖生理と成長因子" メジカルビュー社, 207 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Iwashita: "Fetal growth and growth factor" Perinatal cave. 165. 81-86 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Iwashita: "IGF-I and fetal growth" Hormone Frontier. 2. 57-61 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Iwashita: "Physiological siguificance of IGF-I and its binding proteins in fetal growth and maturation" Acta obstetrica et Gynaecologica Japonica. 48. 659-715 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Iwashita: Reproductive physiology and growth factor. S.Sakamoto, Y.Taketani and H.Minakuchi (eds.) in Obstetrical gynecologycal view 15. Medical View Co., 168-180 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 岩下 光利: "胎児発育・成熟におけるIGF-Iとその結合蛋白の意義" 日本産科婦人科学会雑誌. 46. 659-715 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岩下 光利: "胎児発育と成長因子" ペリネイタルケア. 165. 81-86 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岩下 光利: "胎児発育とIGF" 内分泌・糖尿病科. 1. 459-468 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岩下 光利: "IGF-Iと胎児発育" HORMONE FRONTIER. 2. 57-61 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岩下 光利: "生殖生理と成長因子" メジカルビュー社, 207 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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