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Apoptosis in female genital organs-the relation between bcl-2 protein and hormone receptor

Research Project

Project/Area Number 06671696
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionOsaka Medical College

Principal Investigator

OTSUKI Yoshinori  Osaka Medical College, Professor, 医学部, 教授 (50140166)

Co-Investigator(Kenkyū-buntansha) TUJIMOTO Yoshihide  Osaka University College, Professor, 医学部, 教授 (70132735)
AKAO Yukihiro  Osaka Medical College, Associate Professor, 医学部, 助教授 (00222505)
杉本 修  大阪医科大学, 医学部, 教授 (00084822)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsApoptosis / Endometrium / bcl-2 / ER / PgR / Menstruation / bcl-2蛋白 / IL-7存在性B細胞株 / TUNEL法 / 免疫電顕 / ウエスタン・ブロット / ホルモンリセプター
Research Abstract

Menses is generally regarded as ischemic necrosis of functional layr caused by contraction of spiral arteries, depending on sexual hormone levels. The recent electron-microscopic study, however, demonstrated the presence of apoptotic bodies mainly in the late secretory phase. It is well known that bcl-2 has an ability to block apoptosis in various cell lineages. Therefore, uterine endometrium is an attractive tissue for studying roles of bcl-2 protein during tissue reconstruction and also regulation of bcl-2 expression by hormones. This study was designed to clarify the distribution of bcl-2 protein in human uterine samples throughout menstrual cycle, in comparison with the distribution of estrogen receptor (ER) and progesterone receptor (PgR), and a role of bcl-2 protein in the modulation of apoptosis during menstruation. Uterine samples were collected from 21 women undergoing hysterectomy with informed consent and were obtained from the fundus within the uterine cavity and examined via immunohistochemistry and Western blot analysis for bcl-2 protein, ER and PgR.Bcl-2 protein was observed in human endometrial glandular, stromal and myometrial smooth muscle cells. Especially, glandular cells expressed bcl-2 protein at proliferative through early secretory phases but not at late secretory through menstrual phases. This cyclic expression of bcl-2 was similar to that of ER or PgR ; bcl-2 expression in glandular cells may be regulated by ovarian hormones. The disappearance of bcl-2 expression in glandular cells at late secretory phase was ocnsistent with the appearance of apoptotic cells at the same phase. Therefore, bcl-2 protein may play an important role in the modulation of apoptosis during menstruation.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Yukihiro Akao et al.: "Multiple subcellular localization of bcl-2: Detection in nuclear outer membrane, endoplasmic reticulum membrane, and mitochondrial membranes." Cancer Research. 54. 2648-2671 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshinori Otsuki et al.: "Cyclic bcl-2 expression in human uterine endometrium during menstrual cycle." The Lancet. 344. 28-29 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chiharu Inoki et al.: "Apoptosis in human endometrium during menstrual cycle-ultrastructural detection and image analysis of DNA strand breakes by in situ end-labeling techniques-" J Pathology. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yukihiro Akao et al.: "Multiple subcellular localization of bcl-2 : Detection in nuclear outer membrane, endoplasmic reticulum membrane, and mitochondrial membranes." Cancer Research. 54. 2648-2471 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshinori Otsuki et al.: "Cyclic bcl-2 expression in human uterine endometrium during menstrual cycle." The Lancet. 344. 28-29 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chiharu Inoki et al.: "Apoptosis in human endometrium during menstrual cycle -ultrastructural detection and image analysis of DNA strand breakes by in situ end-labeling techniques-" J.Pathology. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大槻 勝紀: "ヒト子宮内膜および子宮線筋症におけるアポトーシス-特にアポトーシス関連遺伝子蛋白の発現について-" エンドメトリオーシス研究会会誌. 16. 1-5 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] C. Inoki: "Apoptosis in human endometrium durig menstrual cycle-Ulutrastructural detection and image analsys of DNA strand breakes by in situ end-labeling techniques-" J. Pathology. in press. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Akao,Y.Otsuki,S.Kataoka,Y.Ito and Y.Tsujimoto: "Multiple subcellular localization of bcl-2: Detection in nuclear outer membrane, endoplasmic reticulum membrane,and mitochondrial membranes" Cancer Res.54. 2468-2471 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Otsuki,O.Misaki,O.Sugimoto,Y.Ito,Y.Tsujimoto and Y.Akao: "Cyclic bcl-2 gene expression in human uterine endometrium during menstrual cycle" The Lancet. 344. 28-29 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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