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Study for the calcium extrusion mechanism in pregnant human myometrium.

Research Project

Project/Area Number 06671698
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionFukuoka University

Principal Investigator

KAWARABAYASHI Tatsuhiko  School of Medicine, Professor, 医学部・産婦人科, 助教授 (30142350)

Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Keywordshuman myometrium / potassium contracture / sodium-free contracture / Na-Ca exchange / Nifedipine / Ca-ATPase inhibitor / Caポンプ / 子宮筋 / 単離細胞 / 細胞内Ca / オキシトシン / ベラパミル / ライアノジン / Fura-2 / プロスタグランジンF_<2α> / プロスタグランジンE_2 / Ca排出機構 / Mgイオン
Research Abstract

Periodic uterine relaxation in labor relieves the fetus from hypoxia related to sustained contractions. A reduction in intracellular calcium is essential for relaxation. Therefore we aimed to clarify the extrusion mechanisms of intracellular calcium ions in pregnant human myometrium.
Isometric contraction of small muscle bundles obtained from human myometrium at term was recorded. High-potassium and sodium-free solutions could induce stable contractures that revealed initial phasic contractions followed by tonic contractions. The effects of external calcium, external magnesium, nifedipine, and calcium-ATPase inhibitors on both contractures were then examined.
Because maximum tonic contraction was induced by 48 mM potassium, this concentration was used. Both contractures depended on external calcium concentrations. Nifedipine, 10^<-7>M inhibited both initial phasic contractions and the tonic phase of the high-potassium contracture ; however, it could not suppress the tonic phase of the sodium-free contracture. Calcium-ATPase inhibitors, cyclopiazonic acid and lauryl sulfate, potentiated the tensions of tonic phases in both contractures ; the effect of lauryl sulfate was stronger than that of cyclopiazonic acid.
When external magnesium was gradually increased from 0 to 9.6 mM,both phasic contractions and the tonic phase of the high-potassium contracture transiently increased, followed by a reduction of tension, whereas the tonic phase of the sodium-free contracture was markedly inhibited by magnesium in a dose-dependent manner.
These results suggest that the presence of the sodium-calcium exchange mechanism and the specific inhibitory effect of magnesium were indicated in human myometrium during pregnancy. These mechanisms might prevent the long tonic contractions, to protect the fetus from hypoxia during pregnancy and parturition.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Morishita,F.,Kawarabayashi,T.,Sakamoto,Y.,Shirakawa,K.: "Rde of the sodium-calcium exchange mechanism and the effects of magnesium on sodium-free and high-potassium contractures in pregnant human myometrium." Am. J. Obstet. Gynecol.172. 186-195 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Miyata,S.,Lin,S-H.,Kawarabayashi,T.,ea al.: "Lactation and maintenance of ultrastructural plasticity of the supraoptic nucleus in the ovariectomized rat." Brain Res. Bull.37. 405-409 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kaneko,Y.,Kawarabayashi,T.,Sugimori,H.,Tsukamoto,T,: "Changes in Kinetic properties of oxytocin receptors in longitudinal muscle membranes of rat uterus during gestation." J. Mol. Recog.8. 179-183 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawarabayashi,T.,Kobayashi,M.,Akahane,M.,Ajisawa,Y,: "Comparison of in vitro and in vivo inhibitory effects of peptide and nonpeptide oxytocin antagonists on radioligand binding and uterine contractility of ratsduring pregnary." Am. J.Obstet. Gynecol.175. 1348-1355 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawarabayashi,T.,Shojo,H.,Nakamura,S.,Sugimori,H.,et al.: "changes in responsiveness of freshly isolated longitudinal muscle cells from rat uterus towards oxytocinduring gestation : contractility and calcium" Mol. Cell. Endocrinol。. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakao,K.,Inoue,Y.,Okabe,K.,Kawarabayashi,T.,Kitamura,K.,: "Various effects of oxytocin on membrane excitability in pregnant human myometrium." Am. J.Obstet. Gynecol.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawarabayashi,T.: "Electrophysiology of the human myometrium. In : The Vterus" Cambridge University Press, 385 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morishita, F., Kawarabayashi, T., et al.: "Role of the sodium-calcium exchange mechanism and the effect of magnesium on sodium-free and high-potassium contractures in pregnent human myometrium." Am. J.Obstet. Gynecol.172. 186-195 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] miyata, S., Lin, S.-H., Kawarabayashi T., et al.: "Lactation and maintenance of ultrastructural plasticity of the supraoptic nucleus in the overiectomized rat." Brain Res. Bull.37. 405-409 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kaneko, Y., Kawarabayashi, T., et al.: "Changes in kinetic properties of oxytocin receptors in longitudinal muscle membranes of rat uterus during gestation." J.Mol. Recog.8. 179-183 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Lin, S.-H., Miyata, S., Itoh, T., Kawarabayashi, T., et al.: "Fos expression in the hypothalamic magnocellular neurons of rats during pregnancy, parturition and lactation." Neurosci. Res.23. 29-34 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Lin, S.-H., Miyata, S., Kawarabayashi, T., et al.: "Hypertrophy of oxytocinergic magnocellular neurons in the hypothalamic supraoptic nucleus from gestation to lactation." Zool. Sci.13. 161-165 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T,Kaneko, Y., Kawarabayashi, T., et al.: "Characteristics of rat uterus longitudinal muscle beta-adrenergic receptors : Changes in kinetic properties of the receptor during gestation." J.Mol. Recog.9. 233-238 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawarabayashi, T., Kobayashi, M., et al.: "Comparison of in vitro and in vivo inhibitory effects of peptide and nonpeptide oxytocin antagonists on radioligand binding and uterine contractility of rats during pregnancy." Am. J.Obstet. Gynecol.175. 1348-1355 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawarabayashi, T., Shojo, H., et al.: "Effects of oxytocin, prostaglandin F_<2alpha> and prostaglandin E_2 on intracellular free calcium concentrations of longitudinal muscle cells isolated from term pregnent rat myometrium." Gynecol. Obstet. Invest.(in press.). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawarabayashi, T., Shojo, H., et al.: "Changes in responsiveness of freshly isolated longitudinal muscle cells from rat uterus towards oxytocin during gestation : contractility and calcium signaling." Mol. Cell. Endocrinol.(in press.). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakao, K., Inoue, Y., Okabe, K., Kawarabayashi, T., et al.: "Various effects of oxytocin on membrane excitability in pregnant human myometrium." Am. J.Obstet. Gynecol.(in press.). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Lin,s-H.,Miyata,S.,Kawarabayashi.T.,Nakashima,T.,Kiyohara,T.: "Hypertrophy of oxytocinergic magnocellular neurons in the hypothalamic supraoptic nucleus from gestation to lactation." Zool.Sci.13. 161-165 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kaneko,Y.,Kawarabayashi,T.,Ikeda,M.,Sugimori,H.,Tsukamoto,T.: "Characteristics of rat uterus longitudinal muscle B-adrenergic receptors : Changes in Kinetic properties of the receptor during gestation." J.Mol Recog. 9. 233-238 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kawarabayashi,T.,Kobayashi,M.,Akahane,M.,Ajisawa,Y.: "Comparison of in vitro and in vivo inhibitory effects of peptide and nonpeptide oxytocin antagonists on radioligand binding and uterine contractility of rats during pregnancy." Am.J.Obstet.Gynecol.175. 1348-1355 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kawarabayashi,T.,Shojo,H.,Tsukamoto,T.: "Effects of oxytocin,prostaglandin F2α and prostaglandin Ee on intracellular free calcium coneentrations of longitudinal muscle cells isolated from term pregnant rat mycmetrium" Gynecol.Obstet.Invest.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kawarabayashi,T.,Shojo,H.,Nakamura,S.,Sugimori,H,.Tsukamoto,T.: "Changes in responsiveness of freshly isolated longitudinal muscle cells from rat uterus towards oxytocin during gestation : contractility and calcium signaling" Mol.Cell.Endocrinol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakao,K.,Inoue,Y.,Okabe,K.,Kawarabayashi,T.,Kitamura,K.: "Various effects of oxytocin on membrane excitability in pregnant human myometrium." Am.J.Obstet.Gynecol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Miyata, S., Lin, S. -H., Kawarabayashi, T. et al.: "Lactation and maintenance of ultrastructural plasticity of the supnaoptic nucleus in the ovariectomized rat." Brain Res. Bull.37. 405-409 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kaneko, Y., Kawarabayashi, T., Sagimori, H., Tsukamoto, T.: "Changes in kinetic properties of oxytocin receptors in longitudinal muscle membranes of rat uterus during gestation." J. Mol. Recog.8. 179-183 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Lin, S.-H., Miyata, S., Itoh, T., Kawarabayashi, T., et al.: "Fos expression in the hypothalamic magnocellular neurons of rats during pregnancy, parturition and lactation." Neurosci. Res.23. 29-34 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsukamoto, T., Shojo, H., Yamaguchi, K., Kawarabayashi, T., et al.: "Effects of various stimulants on intracellular free calcium concentration of longitudinal muscle cells isolated from pregnant rat myometrium." J. Smooth Muscle Res.in press (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 松尾直裕,瓦林達比古,京野和夫 その他: "超低出生体重児の短期および長期予後に関する臨床的検討." 福大医紀. (in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kaneko, Y., Kawarabayashi, T., Ikeda, M., et al.: "Characteristics of rat uterus longitudinal muscle β-adrenergic receptors : changes in kinetic properties of the receptor during gestation." J. Mol. Recog.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Morishita,F.,Kawarabayashi,T.,et al.: "Role of the sodium-calcium exchange mechanism and the effect of magnesium on sodium-free and high-potassium contractures in pregnar human myometrium." American Journal of Pbstetrics and Gynecology. 172. 186-195 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miyata,S.,Lin,S-H.,Kawarabayashi,T.,et al.: "Lactation and maintenance of ultrastructural plasticity of the supraoptic nucleus in the ovariectomized rat." Brain Reseach Bulletin. (in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kaneko,Y.,Kawarabayashi,T.,et al.: "Changes in the kinetic properties of oxytocin receptors in longitudenal muscle membranes of the rat uterus during gestation." Journal of Molecular Recognition. (in press.). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kaneko,Y.,Kawarabayashi,T.,et al.: "Binding of [^3H]dihydroalprenolol to longitudinal muscle membranes of late pregnant rat myometrium." Journal of Smooth Muscle Research. (in press.). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 森下富士夫,辻岡寛,馬渡秀仁,瓦林達比古,坂本康二,白川光一。: "妊娠ヒト子宮筋におけるNa-Ca交換機構の役割および除Na拘縮ならびに高K拘縮におけるMgの効果。" Journal of Smooth Muscle Research. (in press.). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 池田雅彦,杉本甫,瓦林達比古,松岡由希子,塚本卓治.: "ラット子宮平滑筋におけるGap junctionの形成過程について" Journal of Smooth Muscle Research. (in press.). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawarabayashi,T.: "Electrophysiology of the human myometrium.In:The Uterus" Cambridge University Press., 385 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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