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Regulation of Sex Steroid Receptor Gene Expression in Cultured Hrman Endometrial Cells

Research Project

Project/Area Number 06671699
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionTazuke Kofukai Medical Research Institute

Principal Investigator

IWAI Masazumi  Tazuke kofukai Medical Res.Insti., 医学研究所・第2研究部, 研究員 (20240822)

Co-Investigator(Kenkyū-buntansha) FUJITA Jun  Dep.of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 医学部, 教授 (50173430)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
KeywordsSex Steroid / Receptor / Progesterone / Testosterone / Endometrial Stromal Cells / Gene Expression / Testosterone / Endometrial Stromal Cells. / 子宮内膜 / 性ステロイド / 性ステロイド受容体 / プロゲステロン / テストステロン / PCR
Research Abstract

Sex steroid is known to regulate sex steroid receptors in uterine cells. However, its precise regulation at the mRNA level is unclear. In this study we examined the efect of progesterone (P), estrogen (E), and testosterone (T) on the regulation of sex steroid rceptors in cultured human endometrial stromal cells (ESC) using quantitative reverse transcriptase polymerase chain reaction (RT-PCR) method.
We isolated ESC from human endometrial tissues, and cultured them with or without sex steroid. Incubation with P decreased progesterone receptor (PR), estrogen receptor (ER), and androgen receptor (AR) mRNA levels in cultured human ESC to 0.56,0.53, and 0.84-fold, respectively. T also decreased PR,ER,and AR mRNA levels in cultured human ESC to 0.48,0.52, and 0.82-fold, respectively. on the other hand, E increased PR,ER,and AR mRNA levels in cultured human ESC to 3.0,1.3, and 1.3-fold, respectively. We also examined the sex steroid receptor levels in human ESC cultured for 0,3,6, and 9 days. The PR mRNA level in ESC without P was increased in a time dependent manner. This increase was also inhibited by P and the mRNA levels in the presence of P was almost constant throughout the culture periods.
Our results demonstrated that P,E or T is a regulator of sex steroid receptors in ESC and that this regulation may influence the responsiveness to P in decidual change of ESC.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] M. Iwai: "Regulation of Sex Steroid Receptor Gene Expression by Progesterone and Testosterone in Cultured Humman Endometrial Stromal Cells." J Clin Endocri Metab. 80. 450-454 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Higuchi: "Expression of messenger ribonucleic acid for gonadal steroid receptors in the human pelvic peritoneum." Fertil Steril. 63. 52-57 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Higuchi: "Induction of Tissue Inhibition of Metalloproteinase 3 Gene Expression during in Vitro Decidualizntion of Human Endometrial Stromal Cell." Endocrinology. 136. 4973-4981 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iwai M,Kanzaki H,Fujimoto M,Kojima K,Hatayama H,Inoue T,Nakayama H,Mori T,and Fujita J.: "Regulation of Sex Steroid Receptor Gene Expression by Progesterone and Testosterone in Cultured Human Endometrial Stromal Cells" J Clin Endocri Metab. 80. 450-454 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Higuchi T,Kanzaki H,Iwai M,Narukawa S,Fujita J,Mori T: "Expression of messenger ribonucleic acid for gonadal steroid receptors in the human pelvic peritoneum" Fertil steril. 63. 52-57 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Higuchi T,Kanzaki H,Nakayama H,Fujimoto M,Hatayama H,Kojima K,Iwai M,Narukawa S,Mori T,Fujita J.: "Induction of Tissue Inhibition of Metalloproteinase 3 Gene Expression during in Vitro Decidualization of Human Endometrial Stromal Cell" Endocrinology. 136. 4973-4981 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Iwai: "Regulation of Sex Steroid Receptor Gene Expression by Progesterone and Testosterone in Cultured Human Endometrial Stromal Cells." Journal of Clinical Endocrinology and Metabolism.80. 450-454 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Higuchi: "Expression of messenger ribonucleic acid for gonadal steroid receptors in the human pelvic peritoneum." Fertility and Sterility. 63. 52-57 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Higuchi: "Induction of Tissue Inhibitor of Metalloproteinase 3 Gene Expression during In Vitro Decidualization of human Eudometrial Stromal Cells" Endocrinology. 136. 4973-4981 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kojima K.: "Expression of leukemia inhibitory factor (LIF) in human endometrium and placenta." Biology of Reproduction. 50. 882-887 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hatayama H.: "Progewterone induce Macrophage Colony-Stimulating Factor(M-CSF) Production in the Human Endometrial Stromal Cells in vitro." Endocrinology. 135. 1921-1927 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yokoi H: "High incidence of uterine inversion in mast cell-deficient osteopetrotic mutant mice of mi/mi genotype." Biology of Reproduction. 50. 1034-1039 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Inoue T.: "Tumor necrosis factor a inhibits in-vitrodecidualization of human endometrial stromal cells." Human Reproduction. 9. 2411-2417 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kanzaki H.: "Human endometrial function and the immune system." The proceedings of the VIII World Congress on Human Reproduction.321-324 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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