| Project/Area Number |
06671710
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Mie University |
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Principal Investigator |
SHIMIZU Takeshi M.D.Department of Otorhinolaryngology, Mie Univ.Faculty of Medicine, Assistant, 医学部, 助手 (00206202)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Monoclonal antibody / Chronic sinusitis / Nasal allergy / Goblet cell / Mucus / Nasal gland / Nasal epithelium / Mucus secretion / ELISA |
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| Research Abstract |
A series of monoclonal antibodies were generated against nasal secretions from patients with chronic sinusitis. Four different types of eighteen monoclonal antibodies were obtained. Antibody HCS 18 reacted with epithelial goblet cells. Antibody HCS 4,5,6, and 16 stained submucosal gland cells. Antibody HCS 13 and 15 reacted with epithelial goblet cells, submucosal gland cells and endothelial cells of vessels. Other eleven antibodies recognized epithelial goblet cells and submucosal gland cells. Periodate sensitivity of antigens suggested that all antibodies recognized carbohydrate moieties. Western blotting revealed that most of antibodies recognized high molecular weight materials, but two antigens were denatured by SDS and heat treatment. Cross-reactivity of these antibodies with secretory cells in other organs and in other species were also determined.
The different reactivities with these antibodies were observed in dot blot analysis of pooled nasal secretions from patients with chronic sinusitis and allergic rhinitis. Immunohistological studies of nasal epithelium also revealed the disease-specific changes of nasal secretions in chronic sinusitis, allergic rhinitis, and postoperative maxillary cyst.
We have developed an air-liquid interface in vivo cell culture system to study the mechanisms of mucus secretion, and goblet cell-specific antibody HCS 18 is very useful to quantify the amount of mucus secretion in this culture system.
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