| Project/Area Number |
06671724
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Otia Medical University |
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Principal Investigator |
KURONO Yuichi Oita Medical University, Dept.of Otolaryngology, Assistant Professor, 医学部, 講師 (80153427)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGEMI Hideo Oita Medical University, Dept.of Otolaryngology, Instructor, 医学部, 助手 (50271135)
MOGI Goro Oita Medical University, Dept.of Otolaryngology, Professor, 医学部, 教授 (20035190)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Haemophilus influenzae / Mucosal immunity / Upper respiratory tract infection / Otitis media / Nasopharynx / Bacterial adherence / Outer membrane protein / Oral vaccination |
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| Research Abstract |
1)Detection of Haemophilus inflenzae by PCR :
PCR was applied to detect H.influenzae from middle ear effusion of patients with otitis media with effusion. The incidence of P6-DNA was significantly higher than that of bacteria detected by conventional culture method. P6-DNA was also frequently found in adenoids. The results suggest that presence of H.influenzae in middle ear effusion and in the nasopharynx is more common than previously reported.
2)Mucosal immune responses in the nasopharynx and adenoids :
Secretory IgA antibody activities in nasopharyngeal secretions of patients with otitis media with effusion were determined by ELISA.The antibody titers against H.influenzae were inversely correlated with the number of H.influenzae in the nasopharynx. ELISPOT assay of adenoid lymphocytes showed the presence of IgA and IgG antibody secreting cells specific to outer membrane proteins of H.influenzae. The number of lymphocytes were inversely correlated with the number of H.influenzae in aden
… More
oids. The result suggest that nasopharyngeal colonization of H.influenzae might be inhibited by secretory IgA in the nasopharynx, and that adenoids might regulate mucosal immune responses in the nasopharynx.
3)Effects oral immunization with H.influenzae :
Oral administration of formalin-killed nontypeable H.influenzae or outer membrane proteins with adjuvant increased murine IgA antibody activities in saliva, nasopharyngeal secretion and serum. ELISPOT assay showed that outer membrane-specific IgA secreting lymphocytes were significantly increased in nasopharyngeal mucosa as well as in intestinal lamina propria after oral immunization. Moreover, live H.influenzae inoculated into the nasopharynx were rapidly eliminated in mice having oral immunization. The results suggest that oral immunization enhanced mucosal immunity and the clearance of the nasopharynx, and that oral immunization with H.influenzae might be effective in preventing upper respiratory tract imfection such as otitis media and sinusitis. Less
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