| Project/Area Number |
06671733
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Tokai University School Medicine |
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Principal Investigator |
ANDO Asako Tokai University School of Mediciine, Lecturer, 医学部, 講師 (40101935)
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| Co-Investigator(Kenkyū-buntansha) |
HORIUCHI Masatoshi Tokai University School of Mediciine, Assistant Professor, 医学部, 助教授 (30157060)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | HLA / salivary gland tumor / INT3 / cDNA clone / translocation / microsatellite / polymorphism / repeats sequence / Notch3 / int-3 / 発がん遺伝子 |
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| Research Abstract |
We have identified a human homologue of the mouse int-3 gene, INT3 located on the junction of the class II abd class III regions of the human major histocompatibility complex (MHC) , the neighboring region of a chromosome aberration in salivary gland tumor and suspected that this INT3 gene is acandidate gene responsible for the development of salivary gland tumor. INT3 cDNA clones were isolated and sequenced. Analyzes of the nucleotide sequences of the INT3 cDNA clones suggested that the INT3 gene was composed of the intracelluar portion of Notch-homologous genes and the extracellular portion of the transmembrane region, Notch/lin-12 repeats, and EGF-like repeats. In the the 5'-upstream region of the INT3 gene, (CTG) n repeats showed microsatellite polymorphism with various numbers of repeats for different HLA haplotypes. Five alleles have been identified in 66 HLA homozygous B-lymphoblastoid cell lines. To ascertain if (CTG) n repeats polymorphism may correlate with salivary gland tumor, polymorphism of the (CTG) n repeats have been analyzed in 19 samples of salivary gland tumor. Four alleles have been identified as well as in a Japanese population. No expansion or new alleles of this repeats were found in this neoplasia. In this neoplasia, no large-scale deletion or translocation, either was detected using genomic Southern hybridization analysis by the INT3 cDNA insert as a probe.
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