• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of a Pathogenic Streptococcal Antigen in Behcet's disease

Research Project

Project/Area Number 06671746
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionHokkaido University

Principal Investigator

KOTAKE Satoshi  Hokkaido University, University Hospital, Lecturer, 医学部附属病院, 講師 (00186694)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Koji  Hokkaido University, School of Medicine, Instructor, 医学部, 助手 (00250431)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsBehcet's disease / Streptococcus / Uveities / Cloning / 免疫反応
Research Abstract

To analyze immunopathologic mechanisms of Behcet's disease, we cloned and sequenced the gene encoding a streptococcal antigen correlated with this disease. Cellular DNA of Streptococcus (S.) sanguis KTH-1 strain was extracted and digested with EcoRI.The fragments were cloned by using bacteriophage lgtl 1 and Escherichia coli. DNA library of Eco RI fragments was plated and immunoscreened with the serum antibody of a patient with Behcet's disease, who showed a high antibody value to KTH-1 strain. The DNA fragment isolated from immunopositive clones consisted of about 1.5kbp, and then was subcloned into pUC118 plasmid. The nucleotide sequnce of this clone reveled thant the 3'-terminal region of the insert contained a 950bp opne reading frame (ORF) discontinued by digestion with EcoRI.The molecular weight of this region was equal to that of the streptococcal antigen which responsed to serum antibody of the same patient by means of Western blotting. In an attempt to decide the nucleotide sequence of the 3'-terminal region of this ORF,the polymerase chain reaction was employed after ligation of appropriate restriction fragments and cassettes. The entire ORF was starting at ATG (position 603) which was proceeded by Shine-Dalgarno ribosome binding site, and was capable of coding a peptide 2,550 amino acid residues with a calculated molecular mass of about 94,800Da. The amino acid sequence is homologous to that of Brn-3b with 60% identical residues.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 吉川浩二: "ベーチェット病と連鎖球菌抗原" 日眼会誌. 100. 173-180 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kotake, S.: "Clinical Study on patients with Behcet's disease in Hokkaido" Ocular Immunol Inflamm. 3. 237-241 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yokota, K.: "Characterization of Streptococcus sanguis isolated from patients with Behcat's disease" Microbiol Immunol. 39. 729-732 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 松田英彦: "ぶどう膜炎-最近の知見" 日本医事新報. 3735. 15-20 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小竹聡: "自己免疫疾患に対する新たな治療の可能性" 白眼会誌. 98. 121-129 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshikawa, K: "Behcet's disease and Streptococcal antigen" J Jpn Ophthalmol Soc. 100. 173-180 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kotake, S: "Clinical study on patients with Behcet's disease in Hokkaido" Ocular Immunol Inflamm. 3. 237-241 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yokota, K: "Characterization of Streptococcus sanguis isolated from patients with Behcet's disease" Microbiol Immunol. 39. 729-732 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuda, H: "Uveitis-Recent topics" Nipponijishinpo. 3735. 15-20 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kotake, S: "New approachs to the regulation of atoimmune disease" J Jpn Ophthalmol Soc. 98. 121-129 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 吉川浩二: "ベーチェット病と連鎖球菌抗原" 日眼会誌. 100. 173-180 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kotake,S.: "Clmical Study on patients with Bohcat's disease in Hokkaido" Ocular Jnmunol.Inflamm.3. 193-195 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi