| Project/Area Number |
06671767
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyushu University |
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Principal Investigator |
ISHIBASHI Tatsuro Kyushu University, Faculty of Medicine Associate Professor, 医学部, 助教授 (30150428)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | New vessels / Neovascularization / Endothelial cells / Pericytes / In vitro / In vivo / Retinal vessels |
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| Research Abstract |
1.Pericytes of newly formed vessels in experimental choroidal neovascularization.
We examined the role of pericytes in the progression of choroidal neovascularization in a primate model. Choroidal neovascularization was induced by intense laser photocoagulation in monkey eyes. The eyes were enucleated and were studied with electron microscopy. Pericytes were involved maturation of the endothelial cells that formed choroidal new vessels.
2.Effect of tecogalan sodium on angiogenesis in vitro by choroidal endothelial cells.
We examined the possible inhibitory effect of tecogalan sodium on three important components of in vitro angiogenesis (endothelial proliferation, migration, and tube formation) using bovine choroidal endothelial cells (CECs). Basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and a combination of bFGF and VEGF stimulated proliferation, migration, and tube formation by CECs in vitro. These stimulatory effects were inhibited by tecogalan sodiu
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3.Tecogalan sodium inhibits comeal neovascularization induced by basic fibroblast growth factor.
The antiangiogenic effect of tecogalan sodium on corneal neovascularization was investigated. Comeal neovascularization of rabbits induced by bFGF was inhibited by tecogalan sodium in a dose-dependent manner.
4.Vessel formation by choroidal endothelial cells in vitro is modulated by retinal pigment epithelial cells.
We elucidated the mechanism of bovine CEC angiogenesis and, in particular, the role of retinal pigment epithelial (RPE) cells by use of an in vitro coculture assay system. RPE cells showed to have angiogenic and antiangiogenic effects on CECs. VEGF anf bFDF playd an important role in this phenomenon.
5.Inhibitory effect of vitamin E succinate on the proliferation of cultured bovine choroidal endothelial cells.
We reported the effect of vitamin E (VE) succinate on the proliferation of cultured bovine CECs. VE succinate was found to significantly inhibit CEC proliferation. The protein kinase C (PKC) stimulator phorbol ester reduced the VE succinate-induced inhibitation od bovine CEC proliferation. PKC was involved in this action at least in part. Less
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