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Midkine in retinal deqeneration of the rat

Research Project

Project/Area Number 06671770
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionKagoshima University

Principal Investigator

UNOKI Kazuhiko  Kagoshima University, Medicine, University Hospital, Assistant Professor, 医学部・附属病院, 講師 (60193926)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsrat / mouse / midkine / bFGF / light damage / ischemia / rescue / retinal degeneration
Research Abstract

We evaluated the protective effects of midkine (MK), the product of a retinoic acid responsive gene, on constant-light induced retinal degeneration in albino Sprague-Dawley rats by anatomical and electrophysiological methods. After one week of constant light exposure, uninjected controls and those injected with phosphate buffer saline (PBS) lost most of the photoreceptor inner and outer segments and the thickness of the ONL was decreased. Eyes that were injected with MK or bFGF demonstrated a significant rescue in the photoreceptor layr with a 2-3 fold increase in the ONL thickness. ERGs were hardly detectable in uninjected eyes after 1 week of constant light. On the other hand, distinct responses were recordable in eyes which had an intravitreal injection with MK and bFGF,and the degree of ERG rescue in terms of the amplitude of b-wave was about 40 to 60% as compared with normal eyes. In the constant light-induced retinal damage of albino BALB/C adult mice, midkine provides a potential agent for the rescue from photoreceptor degeneration. bFGF did not show any effects in the light-damaged photoreceptor degeneration in mice.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Masuda K, Watanabe I, Unoki K, Ohba N and Muramatsu T: "Functional rescue of photoreceptors from the damaging effects of constant light survival-promoting factors in the rat." Invest. Ophthalmol Vis Sci.36. 2142-2146 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okubo A, Sameshima M, Unoki K, Ueghara F: "The ultrastructural study of ribosomes in photorecptor inner segmens of the pcd cerebellar mutantmouse." Jpn. J. Ophthalmol.39. 152-161 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 鵜木 一彦: "細胞成長因子の硝子体注入は視細胞変性の治療に有望か?" 眼科診療プラクティス. 16. 64-65 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 鵜木一彦、大久保明子、有村仁志、上原文行、村松寿子、門松健治、村松喬: "白色マウスの連続光照射網膜変性に対するレチノイン酸応答性成長因子ッドカインの効果" 日本眼科学会雑誌. 99. 636-641 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Unoki K, Ohba N, Arimura H, Muramatsu H and Muramatsu T: "The rescue from the constantinducedretinal degenerationby a retinoic acid geneproducts ; midkine (MK)." Investigative Ophthalmology Visual Science. 35. 4063-4068 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 鵜木一彦: "細胞成長因子の硝子体注入は視細胞変性の治療に有望か?" 文光堂, 2 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Unoki K,Ohba N,Arimura H,Muramatsu H and Muramatsu T: "The rescue from the constantinducedretinal degeneration by a retinoic acid geneproducts ; midkine (MK)." Investigative Ophthalmology Visual Science. 35. 4063-4068 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masuda K,Watanabe I,Unoki K,Ohba N and Muramatsu T: "Functional rescue of photoreceptors from the damagingeffects of constant light by survival-promoting factors in the rat." Investigative Ophthalmology Visual Science. 36. 2143-2146 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Unoki K,Okubo A,Arimura H,Uehara F, Muramatsu H,Kadomatsu K and Muramatsu T: "Beneficiale effects of a retinoic acid geneproducts ; midkine, on constant light-inducedretinal damagrin albino mice." Journal of Japanese Ophthamological Society. 99. 636-641 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okubo A,Sameshima M,Unoki K,Ueghara F: "The ultrastructural study of ribosomes in photorecptorinner segmens of the pcd cerebellar mutantmouse." Japanese Journal of Ophthalmology. 39. 151 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masuda K,Watanabe I,Unoki K,Ohba N and Muramatsu T: "Functional rescue of photoreceptors from the damaging effects of constant light by survival-promoting factors in the rat." Invest.Ophthalmol Vis Sci.36. 2142-2146 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Okubo A,Sameshima M Unoki K, Ueghara F: "The ultrastructural study of ribosomes in photorecptor inner segmens of the pcd cerebellar mutant mouse." Jpn.J.Ophthalmol.39. 152-161 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鵜木 一彦: "眼科診療プラクティス(細胞成長因子の硝子体注入は視細胞変性の治療に有望か?)" 64-65 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Unoki,K: "Rescue of photoreceptors from the damaging effects of constasnt light by midkine,a retinoic acid-responsive gene" Investigative Ophthakmology and visual science. 35. 4063-4068 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 鵜木一彦: "白色マウスの連続光照射網膜変性に対するレチノイン酸応答性成長因子ミッドカインの効果" 日本眼科学会雑誌. (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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