| Project/Area Number |
06671786
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児外科
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| Research Institution | Hokkaido University |
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Principal Investigator |
SASAKI Fumiaki Hokkaido Univ., School of Med., Assistant professor, 医学部附属病院, 講師 (40178661)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Hiromasa Hokkaido Univ., School of Med., Assistant Professor, 医学部, 助手 (30226881)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Neuroblastoma / Topoisomerase I inhibitor / MTT assay / mdr-1 / GST-π |
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| Research Abstract |
Aim : We would like to know the chemosensitivity of topoisomerase I inhibior (CPT-11) against neuroblastoma cells and then examine the factors relating the chemosensitivity such as toposimererase content in the cells and expressino of p-glycoprotein and GST-pi.
Materials and methods : As a topoisomerase In inhibitor CPT-11 (SN-38) and Topotecan provided by pharmaceutica companies were used. Neuroblastoma cell lines such as GOTO,TGW,SK-N-AS,SK-N-BE,SK-N-SH,IMR-32 were used. Chemosensitivity was evaluated with MTT assy and topoisomerase content were measures with indirect flowcytometric assy.
Results : 1) CPT-11 and topotecan were iffective in all neuroblastoma cell lines except SK-N-DZ.2) IC50 of CPT-11 and topotecan was correrated significantly. 3) P-glycoprotein was expressed immunohistochmically in 4/7 cell lines. In other 3 cell lines expressed faintly. No correlation between chemosensitiviy and p-glycoprotein expression was found. 4) GST-pi was expressed in allcell lines. There was no correlation and IC50 of CPT-11.6) Neuroblastoma cells contacted with CPT-11 accumulated to G2・M cell cycle stage on FCM,although content of topoisomerase II did not increase.
Conclusion : Topoisomerase I inhibitor (CPT-11) is effective against neuroblastoma cells. No factors relating chemosensitiviy, such as topoisomerase I inhibitor content and P-glycoprotein and GST-pi expression, was found.
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