| Project/Area Number |
06671789
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
小児外科
|
|---|
| Research Institution | CHIBA UNIVERSITY |
|---|
Principal Investigator |
OHNUMA Naomi Pediatric Surgical Department, Chiba University, School of Medicine, Associate Professor, 医学部, 助教授 (50125910)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IWAI Jun Pediatric Surgical Department, Chiba University, School of Medicine, Assistant, 医学部付属病院, 助手 (70223360)
TANABE Masahiro Pediatric Surgical Department, Chiba University, School of Medicine, Lecturer, 医学部付属病院, 講師 (10207160)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | DAN gene / Neuroblastoma / Suppressor gene / Southern blot / Northern blot |
|---|
| Research Abstract |
The expression of DNA gene (previously designated as NO3 gene) is significantly reduced in a variety of transformed rat fibroblasts, including v-src- (SR-3Y1), SV40 and v-mos-transformed 3Y1 cells, compared with that in parental 3Y1 cells. Recently, DAN gene has been shown to possess a tumor suppressive activity when it is overexpressed in SR-3Y1 cells. To assess the involvement of DAN gene with human neoplasms, we have isolated human DAN counterpart from a normal lung cDNA livrary by using rat DAN cDNA as a probe, and determined its chromosomal location. Human DAN gene mapped to chromosome 1p36.11-p36.13, which is well known to show highly significant linkage with the genesis and/or progression of human neuroblastoma. Southern blot analysis on tumor DNA from 26 patients with neuroblastoma has detected three patients showing genomic rearrangement or deletion within or closely linked to the DAN gene locus. Collectively, we propose that human DAN gene is a possible candidate for a tumor suppressor gene of human neuroblastoma.
|
