• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Charactaristics of alpha-fetoprotein-producing and non-alpha-fetoprotein-producing hepatoblastoma

Research Project

Project/Area Number 06671796
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 小児外科
Research InstitutionShowa University

Principal Investigator

TSUNODA Yuko  Showa University, School of medicine.assistant, 医学部, 助手 (20231441)

Co-Investigator(Kenkyū-buntansha) IIJIMA Tadashi  Showa University, School of Medicine.assistant, 医学部, 助手 (40245832)
KANNO Sohtaroh  Showa University, School of medicine.assistant, 医学部, 助手 (20255730)
SHIBUSAWA Miki  Showa University, School of medicine.assistant professor, 医学部, 助教授 (90138496)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Keywordshepatoblastoma / alpha-fetoprotein / cell line / xenograft / chemosensitivity / hepatocyte growth factor / MTT assay / 生物学的特性
Research Abstract

A six month old girl presented with an abdominal mass, and high serum level of alpha-fetoprotein (AFP). It was diagnosed as a well-differentiated hepatoblastoma by open biopsy. The biopsy specimen was transplanted on a nude mouse, and a xenograft was successfully established.
Characteristics of Hepatoblastoma xenograft
The liver biopsy specimen was transplanted to the back of a BALB/c nude mouse. One month later, a tumor had formed subcutaneously. It was removed, and blood samples were obtained at necropsy. Histologically, the cell structure of the tumor was similar to that of the primary tumor. The tumor's doubling time was 6 days. The serum concentration of AFP in the nude mouse was 98,300 ng/ml, and the percentage of Con A-non-bound AFP was 8%. The mode of the chromosome number was 50, and the phenotype was 50, XX,+der (1q7p) +2+8+20. The DNA of the xenograft was aneuploid. N-myc and c-myc were not detected with high amplication. The avidin-biotin-peroxidase complex method was used for immunohistochemical studies for AFP,alpha-1-antitrypsin and cytokeratin. One third of the carcinoma cells were positive for epidermal growth factor.
Chemosensitivity Test (MTT assay)
The inhibition rates of 5-FU and THP-Ad were almost unchanged at less than 40%, whereas those of CDDP were higher than 60% within the range of doses tested.
Antitumor Effect of Hepatocyte Growth Factor (HGF) on Hepatoblastoma
Scince the xenograft maintained the charactaristics of the original tumor, the effect of HGF on hepatoblastoma xenograft was investigated. The treatment of hepatoblastoma xenograft with recombinant 20 ng/ml HGF produced a marked inhibition of cell growth and a suppression of producing AFP.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Tsunoda Y: "Non-alpha-fetoprotein-producing anaplastic hepatoblastoma cell line" In Vitro Cell Dev.Biol.-Animal. 32. 194-196 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsunoda Y: "Characteristics and chemosensitivity of hepatoblastome xenograft" Showa University Medical Science. 1(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsunoda Y: "Antitumor effect of hepatocyte growth factor on hepatoblastome" Anticancer Res.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsunoda Y., Shibusawa W., Okamatsu T., Yatsuzuka M.and Iijima T.: "Non-alpha-fetoprotein-producing nanplastic hepatoblastoma cell line." In Vitro Cell.Dev.Biol.-Animal. 32. 194-196 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsunoda Y., Shibusawa W., Kabasawa Y., Suzuki J., Gomi A., Yatsuzuka M.and Okamatsu T.: "Characteristics and Chemosensitivity of hepatoblastoma xenograft." Showa University Medical Science. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsunoda Y., Shibusawa M., Tsunoda A.and Okamatsu T.: "Antitumor effect of hepatocyte growth factor on hepatoblastpma." Anticancer Res.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi