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High-resolution electron microscopic study of the initial stage of collagen mineralization.

Research Project

Project/Area Number 06671830
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

MIAKE Yasuo  Tokyo Dental College, Associate Professor, 歯学部, 助教授 (00157421)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsUnusual collagen, / Hydroxiapatite, / Formative disturbance, / High-resolution TEM, / Crystal lattice
Research Abstract

The purpose of this study is to investigate the initial mineralization mechanism of collagen.
Specimens were selected from examples of dentin containing unusual collagen agregates. Initial crystals (about 2nm thick) on normal collagen assumed needle or thin-platelet shape. On the basis of crystal morphology, initial crystals were thought to be made up of hydroxiapatite or octacalcium phosphate.But, since it was impossible to view crystal lattices and micro-area electron diffraction patterns clearly, I was unable to determine crystal type.Mature crystals (about 4nm thick) were platelet-like in shape.Their crystal-lattice spacing (about 0.82nm) indicated hydroxiapatite.Similar results were produced from crystals on unusual collagen agregates.Because unusual collagen agregates form neither periodic striations nor hole zone, their mineralization occurs independent of both these phenomena.Since the direction of the crystal c-axis coincides with that collagen-molecule long axis, it seems that crystal commence depositing lengthwise along collagen molecules.
In conclusion, this study suggests that the initial mineralization of collagen comprises three events : 1) initial precipitation of octacalcium phosphate between collagen molecules, 2) subsequent transformation of octacalcium phosphate into hydroxiapatite, and 3) crystal growth of this hydroxiapatite.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 木代宏: "コルヒチニ投与によるラット切歯象牙芽細胞と象牙質の超微構造的変化" 歯科学報. 96. 141-166 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroshi Kishiro: "Ultrastructural changes in rat-insisor odontoblasts and dentin caused by abministration of colchicine." Shikwa Gakuho. Vol.96 (2). 141-166 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 木代 宏: "コルヒチン投与によるラット切歯象牙芽細胞と象牙質の超微構造的変化" 歯科学報. 96(印刷中). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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