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STUDIES ON THE GUSTATORY SIGNAL TRANSDUCTION MECHANISMS OF MOUSE TASTE CELLS USING CONGENIC CELL LINE

Research Project

Project/Area Number 06671862
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionNagasaki University

Principal Investigator

MIYAMOTO Takenori  Nagasaki University, School of Dentistry, Research Associate, 歯学部, 助手 (10167679)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsMouse Taste Cell / Patch Clamp / Non-Dissociated Cell / Strain Difference / Salt Response / Amiloride / Sweet Response / D-Phenylalanine / D-フェニルアラニン / 剥離上皮 / ルシファー・イエロ- / 膜特性 / アミロライド感受性
Research Abstract

The taste responses to salt-stimuli are known to represent a strain-differences. For example, the salt response of C57BL/6 strain indicates strong amiloride sensitivity, but that of BALB/c does not. we attempted to examine differences of the taste responses among taste cells obtained from these two strains and a congenic strain derived from BALB/c strain. We developed a method to obtain taste responses from non-dessociated taste cells, which are embedded in the taste bud so as to maintain those polarity, using patch clamp technique. This method enable us to perform the localized stimulation to the apical membrane alone. The results is as follows :
(1) Both amiloride-sensitive and -insensitive components are involved in the salt responses of the mouse taste cells. The suppression of K^+ channels was suggested to be one of amiloride-insensitive components.
(2) The salt responses were categorized into three types :
(a) Type I consisted of both amiloride-sensitive and -insensitive components ;
(b) Type II involved amiloride-sensitive component alone ;
(c) Type III involved amiloride-insensitive component alone. Since the higher aversion threshold to NaCl was known to be observed in BALB/c mouse than in C57BL/6, the amiloride-insensitive pathway may relate to the transduction pathway for a different quality of taste from that the amiloride-sensitive pathway does. An involvement of the suppression of K^+ channels, which is one of the sweet taste transduction mechanisms, in the amiloride-insensitive pathway supports this hypothesis. For sweet taste, we have found few number of taste cells responded to D-phenylalanine (D-Phe) so far. In such taste cells responded to D-Phe, both 5'-GMP and saccharin enhanced the response to D-Phe.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Sato,T.,et al.: "Comparison of gustatory transduction mechanisms in vertebrate taste cells." Zool.Sci.11. 767-780 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujiyama,R.,et al.: "Differential distribution of two Ca^<2+>-dependent and -independent K^+ channels throughout receptive and basolateral membranes of bullfrog taste cells." Pflugers Arch.429. 285-290 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 宮本 武典,他: "マウス非単離味細胞からの味応答のホールセル記録" 日本味と匂学会誌. 2. 351-354 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto,T.,et al.: "Whole-cell recording from non-dissociated taste cells in mouse taste bud." J. Neurosci. Meth.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto,T.,et al.: "Properties of Na^+-dependent K^+ conductance in the apical membrane of frog taste cells." Brain Res.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto,T.,et al.: "Amino acid-induced responses in catfish taste cells." J. Exp. Biol.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kurihara,K.,et al.: "Olfaction and Taste XI" Springer-Verlag, 864 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Spielman,A.I.,et al.: "Experimental Cell Biology of Taste and Olfaction" CRL Press, 437 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, T., et al.: "Comparison of gustatory transduction mechanisms in vertebrate taste cells." Zool.Sci.11. 767-780 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujiyama, R., et al.: "Differential distribution of two Ca^<2+>-dependent and -independent K^+ channels throughout receptive and basolateral membranes of bullfrog taste cells." Pflugers Arch.429. 285-290 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto, T., et al.: "Whole-cell recording from non-dissociated taste cells in mouse taste bud." J.Neurosci.Meth.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto, T., et al.: "Properties of Na^+-dependent K^+ conductance in the apical membrane of frog taste cells." Brain Res.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto, T., et al.: "Amino acid-induced responses in catfish taste cells." J.Exp.Biol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Teeter, J.H., et al.: Springer-Verlag (864) Tokyo. In : olfaction and Taste XI.Edited by Kurihara, K., et al., (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto, T., & Sato, T.: CRC Press (437) Boca Raton. In : Experimental Cell Biology of Taste and Olfaction. Edited by Spielman, A.I., et.al., (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 宮本 武典 他: "マウス非単離味細胞からの味応答のホールセル記録." 日本味と匂学会誌. 2. 351-354 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岡田 幸雄 他: "カエル味細胞膜特性に対するバソプレッシン(AVP)の効果." 日本味と匂学会誌. 2. 355-357 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Miyamoto,T.,et.al.: "Whole-cell recording from non-dissociated taste cells in mouse taste bud." J. Neurosci. Meth.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Miyamoto,T.,et.al.: "Properties of Na^+ -dependent K^+ conductance in the apical membrane of frog taste cells." Brain Res.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Miyamoto,T.,et.al.: "Amino acid-induced responses in catfish taste cells." J. Exp. Biol.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Spielman,A.I.,et al.: "Experimental Cell Biology of Taste and Olfaction" CRC Press, 437 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato,T.,et al.: "Comparison of gustatory transduction mechanisms in vertebrate taste cells." Zool.Sci.11. 767-780 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fujiyama,R.,et al.: "Differential distribution of two Ca^<2+> -dependent and -independent K^+ channels throughout receptive and basolateral membranes of bullfrog taste cells." Pflugers Arch.429. 285-290 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 宮本武典 他: "Na^+依存性K^+チャネルのカエル味細胞塩応答への関与" 日本味と匂学会誌. 1. 200-203 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 藤山理恵 他: "カエル味細胞膜におけるカチオンチャネルの特性." 日本味と匂学会誌. 1. 204-206 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 内田昌徳 他: "甘味刺激の味細胞受容器電位への変換の分子機構." 日本味と匂学会誌. 1. 208-211 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Spielman A.I.et al.: "Experimental Cell Biology of Taste and Olfaction" CRC Press(in press), (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kurihara,K.et al.: "Olfaction and Taste XI" Springer-Verlag, 864 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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