| Project/Area Number |
06671890
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | Ohu University |
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Principal Investigator |
YAMASAKI Akira Ohu University, Department of Dentistry, Professor, 歯学部, 教授 (60110459)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Moriaki Ohu University, Department of Dentistry, Assistant, 歯学部, 助手 (70222669)
OHNO Tomoya Ohu University, Department of Dentistry, Professor, 歯学部, 教授 (50094941)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Odontogenic keratocyst / Bone resorption / Immunocompetent cells / Macrophage / Lymphocyte / Immunocytochemistry |
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| Research Abstract |
The distribution of immunocompetent cells in the wall of jaw cysts (15 odontogenic keratocysts and 5 radicular cysts) and the connective tissue capsule of 3 ameloblastomas were examined immunohistochemically.
1.The antibody UCHL-1, which recognized CD4^+/CD8^+T lymphocytes, was intensely expressed in the subepithelial inflammatory lesion of the cyst wall. In the non-inflamed cyst wall, UCHL-1-positive cells tended to be localized in the outermost area adjacent to the bone.
2.KP1 antibody which recognized a wide range of monocyte-macrophage lineage was observed in the perivascular area of fibrous wall as well as inflammatory foci. In the bone tissue, mononuclear cells around the newly formed bone and osteoclasts appeared to expressed this antibody.
3.HAM56 antibody for resident macrophages was observed in the fibrous tissue adjacent to the bone, particularly the cells in the perivascular area. Mononuclear cells in the bone tissue also expressed HAM56 antibody, but they were fewer than KP1-positive cells and no osteoclasts expressed this antibody.
4.MAC387 which recognized leukocytes having antigen L1 was intensely expressed in the inflammatory foci and a part of lining epithelium but no expression was discernible in the fibrous cyst wall and bone tissue.
From the results to date it is presumed that there is a certain relationship between the distribution of immunocompetent cells and growth of the cystic lesion.
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