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Studies on leukocyte function of periodontal desease and diabetes mellitus

Research Project

Project/Area Number 06671896
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionOsaka Dental University

Principal Investigator

OHURA Kiyoshi  Osaka Dent.Univ.Dept.of Pharmacol.Professor, 歯学部, 教授 (20131378)

Co-Investigator(Kenkyū-buntansha) OGATA Keigo  Osaka Dent.Univ.Dept.of Pharmacol.Assistant, 歯学部, 助手 (30214000)
SHINOHARA Mitsuko  Osaka Dent.Univ.Dept.of Pharmacol.Associate Professor, 歯学部, 助教授 (40067187)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsdiabetes mellitus / gingivitis / periodontal disease / rat / leukocyte / macrophage / chemotaxis / phagocytosis / 白血球 / 貧食能 / 歯周炎
Research Abstract

Many studies have shown that periodontal lesions in diabetic patients progress rapidly and resist treatment. Herein, we use the naturally occurring gingivitis rat (ODUS/Odu) with diabetes mellitus induced by injection of streptozotocin (STZ : 65mg/kg, i.v.) and the control rat (ODUS/Odu without STZ). One and 3 months after STZ injection, we intraperitoneally injected liquid paraffin. Four days later, we measured the pocket probing depth (PPD), and collected the blood sample and peritoneal macrophages from these rats. To elucidate the effect of diabetes on periodontal lesions, the chemotaxis and phagocytosis of macrophages were studied in ODUS/Odu with and without diabetes. One and 3 months after STZ injection, the PPD in the ODUS/Odu with diabetes was significantly deeper than in ODUS/Odu without diabetes (p<0.001). PPD was deeper at 3 months after STZ injection than at 1 month in ODUS/Odu with diabetes. Three months after STZ injection, there was a high positive correlation between blood glucose and triglycerides, or hemoglobin A_1C (p<0.01). In both experimental periods, the phagocytosis of macrophages in the diabetes group was significantly more suppressed than in that without diabetes (p<0.001). Three months after STZ injection, the macrophage chemotaxis was more suppressed in ODUS/Odu with diabetes than in those without diabetes. These findings stuggest that diabetes mellitus affects the chemotaxis and phagocytosis of macrophages in ODUS/Odu. Therefore, the host defense mechanism weakens, and worsens periodontal disease.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Mitsuko Shinohara: "Leukocyte function of streptozotocin-induced diabetes in naturally occurring gingivitis rat." XI ICLAS General Assembly and Joint Conference of ICLAS,Scan-LAS and FinLAS. (Abst.). 210 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuko Shinohara: "Influence of streptozotocin diabetes on naturally occurring gingivitis rat." International Conference on Periodontal Research. (Abst.). 61 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuko Shinohara: "Studies on leukocyte function of periodontal disease and diabetes mellitus." 第69回日本薬理学会年会. 予稿集. 67 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Keigo Ogata: "Effect of osmolality and glucose consentration on rat leukocyte functions." Journal of Dental Research. 74. 525 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Keigo Ogata: "Effect of particle size on rat peritoneal macrophage phagocytosis." Japanese Journal of oral Biology. 37. 232-235 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiyoshi Ohura, Keigo Ogata, Mitsuko Shinohara: "Effect of streptozotocin-induced diabetes on leukocyte functions in rat with naturally occurring gingivitis." Canadian Journal of Physiology and Pharmacology. 72. 286 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuko Shinohara, Kiyoshi Ohura: "Leukocyte function of streptozotocin-induced diabetes in naturally occurring gingivitis rat." Scandinavian Journal of Laboratory Animal Science. (in press) (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Keigo Ogata: "Effect of particle size on rat peritoneal macrophage phagocytosis." Japanese Journal of oral Biology. 37. 232-235 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Keigo Ogata: "Effect of osmolality and glucose concentration on rat leukocyte functions." Journal of Dental Research. 74. 525 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuko Shinohara: "Leukocyte function of streptozotocin-induced diabetes in naturally occurring gingivitis rat." XI ICLAS General Assembly and Joint Conference of ICLAS,Scan-LAS and FinLAS Abstract. 210 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuko Shinohara: "Influence of streptozotocin diabetes on naturally occurring gingivitis rat." International Conference on Periodontal Research Abstract. 61 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuko Shinohara: "Leukocyte function of streptozotocin-induced diabetes in naturally occurring gingivitis rat." XI ICLAS General Assembly and Joint Conference of ICLAS, Scan-LAS and FinLAS. Abst.210 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mitsuko Shinohara: "Influence of streptozotocin diabetes on naturally occurring gingivitis rat." International Conference on Periodontal Research. Abst.61 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mitsuko Shinohara: "Studies on leukocyte function of periodontal disease and diabetes mellitus" 第69回日本薬理学会年会. 予稿集. 67 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Keigo Ogata: "Effect of osmolality and glucose concentration on rat leukocyte functions." Journal of Dental Research. 74. 525 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Keigo Ogata: "Effect of particle size on rat peritoneal macrophage phagocytosis." Japanese Journal of oral Biology. 37. 232-235 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Ohura: "Effect of streptozotocin-induced diabetes on leukocyte functions in rats with naturally occurring gingivitis" Canadian Journal of Physiology and Pharmacology. 72. 286- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大浦 清: "実験的歯周炎に関する研究" 日本薬理学会誌. 105. 317-327 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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