Transforming growth factor-beta1 coated beta-tricalcium phosphate pellets stimulate healing of experimental bone defects of rat calvariae
| Project/Area Number |
06671905
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
OKUDA Kazuhiro Niigata University Dental Hospital, Lecturer, 歯学部・附属病院, 講師 (00169228)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Takashi Niigata University School of Dentistry, Associate, 歯学部, 助手 (00251827)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Transforming growth factor beta1 / beta-tricalcium phosphate / Bone formation / Rat / Calvarial defect / Confocal laser scanning microscopy / Alkaline phosphatase / Tartarate-resistant acid phosphatase |
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| Research Abstract |
In an attempt to assess the effect of TGF-beta1-coated beta-TCP pellet grafting on bilateral defects created in rat calvariae, we carried out morphological observations by light microscopy using hematoxylincosin (H-E) staining for histology and detection of alkaline phosphatase (ALPase) and tartrateresistant acid phosphatase (TRACPase) activities for ostcoblastic and osteoclastic cell distribution. Confocal laser scanning microscopy (CLSM) was performed for morphometry of newly formed bone. H-E sections disclosed stronger new bone formation in sites receiving TGF-beta1-coated beta-TCP pellets (experimental sites) than in beta-TCP pellets alone (control sites). On week 4, new bone spots in experimental sites were observed : bone formation occurred from both the periphery and within. The frequency of scquentially arranged TRACPase-positive cells and ALPase-positive cells in experimental sites was higher than in control sites. The bone formation rate calculated by computerized CLSM pixel image analysis confirmed the above findings (9.3(]SY.+-.])1.7% vs. 3.4(]SY.+-.])0.8% on week 2 and 39.8(]SY.+-.])10.9% vs. 11.8(]SY.+-.])2.1% on week 4 ; TGF-beta1-coated beta-TCP pellets vs. beta-TCP pellets). To conclude, TGF-beta1-coated beta-TCP pellet is a recommended synthetic bone graft which promotes bone formation.
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Report
(3 results)
Research Products
(3 results)
