| Project/Area Number |
06671909
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
ARAI Hideo Okayama Univ.Dental Sch., Assistant prof., 歯学部・附属病院, 講師 (70222718)
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| Co-Investigator(Kenkyū-buntansha) |
WASHIO Norifumi Okayama Univ.Dental Sch., Assistant, 歯学部・附属病院, 助手 (40263602)
HONGYO Hiroshi Okayama Univ.Dental Sch., Assistant, 歯学部, 助手 (80243476)
TAKASHIBA Shogo Okayama Univ.Dental Sch., Associate prof., 歯学部, 助教授 (50226768)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Gingival fibroblast / subpopulation / IL-1beta / TNF-alpha / Prostaglandin E_2 / レセプター / 亜群 / PGE_2産生 / コラーゲン合成 |
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| Research Abstract |
Tumor necrosis factor (TNF-alpha) has been known to stimulate prostaglandin production in gingival fibroblasts. In this study, we showed that, by the addition of interleukin-1beta (IL-1beta) into the culture, this enhancement was further augmented. IL-1beta decreased the number of TNF-alpha receptor and affinity to its ligand in gingival fibroblasts. We therefore examined the expression profile of IL-1 receptor mRNA and that of TNF-alpha receptor subtype mRNA under the absence or presence of IL-1beta to understand the underlying mechanisms by which IL-1beta modulate the responsiveness of gingival fibroblasts to TNF-alpha.
Both type I and II TNF-alpha receptor mRNA were detected in gingival fibroblasts, but the expression of type I receptor was much more than that of type II.When gingival fibroblasts was stimulated by IL-1beta, the expression of type I TNF-alpha receptor was markedly depressed, while type II receptor was not affected.
These results indicate that the initial observation that IL-1beta enhances the affinity of TNF-alpha to gingival fibrolasts (leading to increase of PGE_2 synthesis) can not be explained solely by the expression profile of TNF-alpha receptors.
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