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Biological and clinical significance of genetic abnormalities in oral cancer and precancerous lesion.

Research Project

Project/Area Number 06671984
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

CHIBA Itsuo  Hokkaido Univ., Sch. of Dent., Inst., 歯学部, 助手 (50250460)

Co-Investigator(Kenkyū-buntansha) SHINDOH Masanobu  Hokkaido Univ, Sch. of Dent., Inst., 歯学部, 助手 (20162802)
NOTANI Ken-ichi  Hokkaido Univ., Sch. of Dent., Assos. Prof., 歯学部, 助教授 (70113602)
FUJINAGA Kei  Sapporo Med. Univ., Cancer Res. Inst., Dept. of Mol. Biol., Pro., 医学部, 教授 (10045338)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsp53 / Human papillomavirus / Oral squamous cell carcinoma / Prognosis / Betel chewing / Sri Lanka / P53遺伝子 / 口腔癌 / 癌遺伝子 / 腫瘍抑制遺伝子
Research Abstract

We examined 38 primary, resected specimens of oral squamous cell carcinomas (SCCs) for detection of p53 mutations and human papillomavirus (HPV) intection. p53 Mutations were detected in nine cases (24%) using PCR-SSCP analysis. HPV DNA sequences were detected in eight cases (21%) by PCR with HPV-specific primers. Seven cases were p53 mutation-positive/HPV-negative, 6 cases were p53 mutation-negative/HPV-positive, and two intraosseus SCC cases were p53 mutation-positive/HPV-positive. Thus, 15/38 (49%) cases had inactivation of the p53 protein. Interestingly, p53 mutation-negative/HPV-negative cases had a poorer prognosis than p53 nunation positive or HPV-positive cases (p<0.01). We conclude that 1) mutation in tha p53 gene and/or HPV infection are frequent (40%) in oral SCC ; 2) inactivation of p53 function by mutation and HPV infection are important genetic events in the development of 40%- of oral SCCs ; 3) p53 mutation and HPV infection are not mutually exclusive events and 4) other oncogenes or tumor suppressor genes may be crucial in the development of oral SCC if the prognosis is poor. The same type of examinatioin was done for oral SCCs caused by betel chewing in Sri Lanka, where is one of the world's highest incidences of oral SCCs. We found that mutations in the p53 gene of oral cancers in Sri Lanka patients tended to cluster in exon 5. Infections of HPV are now under investigation.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Itsuo Chiba et al.: "Mutations is the p53 gene and human papillomavirus infection as significant prognostic factors in squamous cell carcinomas of the oral cavity." Oncogene. 12, (In press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masanobu Shindoh et al.: "Detection of human papillomavirus DNA sequences in oral squamous cell carcinomas and their relation to p53 and proliferating cell nuclear antigen expression." Cancer. 76. 1513-1521 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Itsuo Chiba et al.: "Characteristics of mutations in the p53 gene of oral squamous cell carcinomas associated with betel quid chewing habit in Sri Lanka." (In preparation).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chiba, I.et al.: "Mutations in the p53 gene and human papillomavirus infection as significant prognostic factors in squamous cell carcinomas of the oral cavity." Oncogene. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shindoh, M.et al.: "Detection of human papillomavirus DNA sequences in oral squamous cell carcinomas and their relation to p53 and proliferating cell nuclear antigen expression." Cancer. 76. 1513-1521 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chiba, I.et al.: "Characteristics of mutations inthe p53 gene of oral saquamous cell carcinomas associated with betel quid chewing habit in Sri Lanka." In preparation.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Itsuo Chiba,et al.: "Mutations in the p53 gene and human papillomavirus infection as significant prognostic factors in squamous cellcarcinomas of the oral cavity" Oncogene. 12(In press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masanobu Shindoh et al.: "Detection of human papillomavirus DNA sequences in oral squamous cell carcinomas and their relation to p53 and proliferating cell nuclear antigen expression." Cancer. 76. 1513-1521 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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