• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analyzes of p16/CDKN2.p53 and ras gene mutations with cell-proliferative activities in oral squamous cell carcinomas and the premalignant lesions

Research Project

Project/Area Number 06672011
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionNara Medical University

Principal Investigator

SUGIMURA Masahito  Nara Medical University, Department of Oral and Maxillofacial Surgery, Professor, 医学部, 教授 (20028749)

Co-Investigator(Kenkyū-buntansha) KITAHORI Yoshiteru  Nara Medical University, 2nd Department of pathology Assistant, 医学部, 助手 (30221211)
KIRITA Tadaaki  Nara Medical University, Department of Oral and Maxillofacial Surgery, Lecturer, 医学部, 講師 (70201465)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsoral tumor / premalignant / cell proliferation / p16 / CDKN2 / p53 / ras / cell proliferation / oral / PCNA / AgNoR / SSCP / premalignant lesions / S.C.C.
Research Abstract

p16/CDKN2, p53 and ras gene mutations were examined using a combination of immunohistochemistry and SSCP-Sequence analyzes with cell proliferative activities such as PCNA and AgNOR in 20 oral squamous cell carcinomas and 20 oral premalignant lesions. The p16/CDKN2 gene mutations were found 2 of 20 cases squamous cell carcinoma, but none of premalignant lesions. Two cases with p16/CDKN2 gene deletions were detected with immunonegative staining for p16 protein. The p53 gene mutations were found 5 of 20 squamous cell carcinoma and none of premalignant lesions. A mutation in the K-ras gene was found in single carcinoma and dysplastic samples.
On the other hand, 35% (7/20) of the oral squamous cell carcinomas demonstrated immunoreactivity for p53 and 50% were immunopositive for ras p21. Two cases with positive staining for p53 in moderate dysplasia and hyperplasia, without p53 gene mutation, were relatively light and seemed to be limited to a few cells within the basal cell layr. PCNA and AgNOR values revealed high scores in these cases, it is suggested that p53 expression may be related to accumulation of wild type protein with rapid cell proliferation rather than gene mutation. The PCNA and AgNOR score tend to be significantly higher in the carcinomas than those in premalignant lesions. From the data, it can be argued that p16/CDKN2 and p53 mutations are relatively late occurrences and that genetic alterations of the ras genes may not play a significant role in human oral tumorigenesis. The tumor suppressor gene such as p16/CDKN2 or p53 seem to be not only independently occurred but involved in later events of oral tumorigenesis.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Hirofumi Matsuda et al.: "Alterations of p16/CDKN2,p53 and ras genes in oral squamous cell carcinomas and premalignant lesions" J Oral Pathol Med. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuda H.Konishi N.Hiasa Y.Hayashi I,Tsuzuki T.Tao M.Kitahori Y.Yoshioka N.Kirita T and Sugimura M: "Alterations of p16/CDKN2.p53 and ras gene mutations in oral squamous cell carcinomas and premalignant lesions" J Oral Pathol Med. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 西岡博人: "口腔扁平上皮癌におけるp53癌抑制遺伝子産物の免疫組織化学的検索:PCNA染色との比較および臨床病理学的関連性について" 日本口腔外科学会雑誌. 39. 201-206 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hiroto Nishioka: "Lmmuohistochemical Detection of p53 Cncoprotein in Human Orul Squamous Cell Correlation and Leukoplakias:Comparison with Proliferating Cell Naclear Antigen Staining and Correlation with Clinicopathelegical Findings" Oncology. 50. 426-429 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Keisuke Horiuchi: "Demonstration of Epstein-Barr Virus in the Oral Epithelial Lesions Using Polymerase Chain Roktion and in situ Hybridization" Oral Oncology. IIIA. 208-211 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tadaaki Kirita: "Risk Factors for the Postoperative Local Recurrence of Tongue Carcinama" J.Oral Maxillofac Surg. 52. 149-154 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 桐田忠昭: "口腔白板症の臨床病理学的検討-特に悪性化潜在能について-" 日本口腔外科学会雑誌. 41. 26-35 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Keisuke Horiuchi: "Epstein-Barr virus in the proliferative diseases of squamous epithelium in the oral cavity" ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY. 79. 57-63 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 杉村正仁: "歯科ジャーナル" 国際医書出版, 731-736 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 杉村正仁: "-歯科医のための全身の見方-(道健一他編集)検査・検査値・全身疾患" デンタルダイヤモンド社, 186-191 (1993)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi