| Project/Area Number |
06672020
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Showa University |
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Principal Investigator |
YAGAMI Kimitoshi Showa University, Dental School, Assistant Professor, 歯学部, 講師 (00210211)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAMAKI Hideaki Showa University, Dental School, Assistant Professor, 歯学部, 助手 (50201520)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Malignant tumor / SOD activity / radiation / anti cancer drugs / tumor cell line / xanthin-xanthin oxidase / progressed cancer / sensitivity / Xanthin-Oxidase / Xanthin Oxidase |
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| Research Abstract |
Recently, many anti cancer drugs have been shown to produce super oxide anion (O_2^-) and seem to involve O_2^- in their mode of action. Ionizing radiation provokes the decomposition reaction of water, producing a variety of reactive oxygen species including O_2^-. The finding that cancer cells are generally low in SOD activity may offer a theoretical base for radiation therapy and chemotherapy. Thus, intracellular SOD level may be an important factor determining the resistance of cancer cells against anti cancer drugs or radiation.
For this purpose, we analyzes the activity of tumor tissue SOD that probed tumor sample form patient at the first time visit, and at the operation. Treatment with anti cancer drugs or radiation a few increasea of SOD activity in tumor tissue. To analyzes the mechanisms of resistance of cancer cells against anti cancer drugs or radiation, xanthin and xanthin oxidase were employed to some tumor cell line as an O_2^- generating system, and treatment with 5-FU or CDDP or ionizing radiation. Treatment with anti cancer drugs or radiation a few increases of SOD activity in tumor cells. The treatment with TNF or INF-gamma did a small reduction of SOD activity in tumor cells. CuZnSOD, catalase and GSH-px mRNAs were up regulated by O_2^- exposure with in 20 minus.
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