ESTABLISHMENT OF EFFICIENT METHOD FOR PRODUCTION OF SCOPADULCIC ACID B BY USING TISSUE CULTURE SYSTEM
| Project/Area Number |
06672092
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
HAYASHI Toshimitsu TOYAMA MEDICAL & PHAR-PHARMACEUTICAL MACEUTICAL RESEARCH UNIVERSITY SCIENCES ASSOCIATE, 薬学部, 助手 (40092796)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Scoparia dulcis / tissue culture / diterpene / scopadulcic acid B / scopadulciol / organ culture |
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| Research Abstract |
1. Effect of light on the production of diterpene after germination of Scoparia dulcis
After the seeds of SDB type S.dulcis were germinated in sterile condition, the effect of light was investigated on the growth of seedlings and the production of scopadulcic acid B (SDB). Under 24 h-light condition, the seedlings grew rapidly 7 days after germination with rapid increase in SDB production. On the other hand, in the dark, the growth of seedlings was suppressed significantly and no SDB was detected even 2 weeks after germination.
2. Effect of plant growth hormones on the growth and diterpene production of leaf organ cultures of S.dulcis
At 4 weeks after germination, the leaves of the seedlings were cut and inoculated on MS liquid medium supplemented with benzyladenin (BA), isopentenyladenin (IP), 4-pyridylurea (4 PU) or kinetin (K). The inocula were subcultured every 2 weeks in the same medium. Resulting cultures were subjected to the determination of growth and diterpene production. When the cultures were analyzed 12 days after subculture, the highest content of the diterpenes was found at the concentration of 0.1 muM of either growth hormone tested. Especially, very high tissue growth and diterpene production was found in the cultures grown in 4 PU-containing medium. In the cultures grown in other growth hormones, significant elongation of roots was observed.
3.Conclusion
Leaf organ culture using MS liquid medium containing 0.1 muM 4PU was found to be the best condition for effective production of SDB by tissue cuture of S.dulcis. It was possible to produce SDB or SDC effi-ciently when leaves of the respective chemotype of S.dulcis. (SDB-type or SDX-type) were used.
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Report
(3 results)
Research Products
(6 results)
