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Development and application of ion-selective electrodes

Research Project

Project/Area Number 06672144
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionOkayama University

Principal Investigator

KATSU Takashi  Okayama Univ., Fac.Pharmaceut.Sci., Associate Professor, 薬学部, 助教授 (40112156)

Co-Investigator(Kenkyū-buntansha) SHINODA Sumio  Okayama Univ., Fac.Pharmaceut.Sci., Professor, 薬学部, 教授 (50029782)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsIon-selective electrode / Potentiometry / Clinical analysis / Therapeutic drug monitoring / Clinical pharmacy / Blood analysis / Forensic chemistry / Drug-membrane interaction / TDM / てんかん / コカイン / 麻薬
Research Abstract

The use of ion-selective electrodes for chemical and medical analyzes continues to be an area of focus. We are interested in applying these electrodes to therapeutic drug monitoring, which is fundamental to pharmacotherapy, as it can be used to adjust the doses of drugs with similar therapeutic and toxic concentration ranges. Here, we introduced new ion-selective electrodes to monitor blood levels of the antiepileptic bromide, the antiarrhythmic procainamide and bretylium, and the anti-inflammatory salicylate. The results correlated well with those obtained by colorimetry, fluorescence polarization immunoassay or gas chromatographic analysis.
For therapeutic drug monitoring, determining the free concentration of a drug is claimed to be physiologically more relevant than that of total concentration of drug (i.e., the sum of the concentration of the free drug and that bound to protein) . Thus, this method is very useful since the electrode is responsive only to free concentration. We compared the free concentrations of salicylate in serum samples determined by a salicylate electrode with those determined by colorimetry. The correlation was good in the salicylate concentration range of 0.1-2.5 mM.The colorimetric method requires deproteinization to estimate the free salicylate concentrations in serum sample, whereas the method using the electrode does not. Thus, the present method is more simple, rapid and economical and, thus, useful for therapeutic drug monitoring in a clinical setting.
We described here only the results of therapeutic drug monitoring. Other results obtained through the present study have also been published (see references) .

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Takashi Katsu: "Ion-selective electrode for serum bromide assay in patients with epilepsy" Clinica Chimica Acta. 234. 157-161 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiyoyuki Watanabe: "Development and application of a cocaine-selective electrode" Japanese Journal of Toxicology and Environmental Health. 41. P-11 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasuhiro Shimonaka: "Changes in the shape of Escherichia coli and human erythrocytes induced by antimicrobial peptides : polymyxin B, EM 49, gramicidin S and tachyplesin" Peptide Chemistry 1994. 69-72 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Katsu: "Ion-selective electrode for procainamide determination in blood serum" Analytica Chimica Acta. 312. 35-38 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiyoyuki Watanabe: "New cocaine-selective membrane electrode" Analytica Chimca Acta. 316. 371-375 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 古野勝志: "小児難治性てんかん患者におけるBr剤投与とその血中濃度測定: 臭素(Br)電極を用いた血清Br濃度の簡便測定と臨床応用" TDM研究. 13. 11-16 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kyoyuki Watanabe: "Development of an amphetamine-selective electrode" Japanese Journal of Toxicolgy and Environmental Health. 42. 33 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Katsu: "Determination of cholinesterase in blood serum using a benzoate-selective membrane electrode made with heptyl-4-trifluoroacetyl-benzoate neutral carrier" Analytica Chimca Acta. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Katsu: "Ion-selective electrode for salicylate assay in blood serum" Talanta. 43 (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Katsu: "A new bretylium-selective electrode for monitoring the drug in blood serum" Analytical Letters. 29 (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Katsu: "Ion-selective electrode for serum bromide assay in patients with epilepsy" Clin.Chim.Acta. 234. 157-161 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiyoyuki Watanabe: "Development and application of a cocaine-selective electrode" Jpn.J.Toxicol.Environ.Health. 41. P-11 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasuhiro Shimonaka: "Changes in the shape of Escherichia coli and human erythrocytes induced by antimicrobial peptides : polymyxin B,EM 49, gramicidin S and tachyplesin" Peptide Chemistry 1994, (Ohno, M., ed) Protein Research Foundation, Osaka. 69-72 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Katsu: "Ion-selective electrode for procainamide determination in blood serum" Anal.Chim.Acta. 312. 35-38 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiyoyuki Watanabe: "New cocaine-selective membrane electrode" Anal.Chim.Acta. 316. 371-375 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katsushi Furuno: "Serum bromide ion concentration in child patients with intractable epilepsy treated with bromide : simple and rapid determination of bromide concentration in serum by potentiometry using a bromide ion-selective electrode" TDM Kenkyu. 13. 11-16 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiyoyuki Watanabe: "Development of an amphetamine-selective electrode" Jpn.J.Toxicol.Environ.Health. 42. P-33 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Katsu: "Determination of cholinesterase in blood serum using a benzoate-selective membrane electrode made with hepty1-4-trifluoroacetylbenzoate neutral carrier" Anal.Chim.Acta. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Katsu: "Ion-selective electrode for salicylate assay in blood serum" Talanta. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Katsu: "A new bretylium-selective electrode for monitoring the drug in blood seum" Anal.Lett.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiyoyuki Watanabe: "New cocaine-selective membrane electrode" Analytica Chimca Acta. 316. 371-375 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 古野 勝志: "小児難治性てんかん患者におけるBr剤投与とその血中濃度測定:臭素(Br)電極を用いた血清Br濃度の簡便測定と臨床応用" TDM研究. 13. 11-16 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takashi Katsu: "Determination of cholinesterase in blood serum using a benzoate-selective membrane electrode made with hepty1-4-trifluoroacetylbenzoate neutral carrier" Analytica Chimca Acta. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takashi Katsu: "Ion-selective electrode for salicylate assay in blood serum" Talanta. 43(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kiyoyuki Watanabe: "Development of an amphetamine-selective electrode" Japanese Journal of Toxicolgy and Environmental Health. 42(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takashi Katsu: "A new bretylium-selective electrode for monitoring the drug in blood serum" Analytical Letters. 29(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takashi Katsu: "Ion-selective electrode for serum bromide assay in patients with epilepsy" Clinica Chimica Acta. 234. 157-161 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kiyoyuki Watanabe: "Development and application of a cocaine-selective electrode" Japanese Journal of Toxicology and Environmental Health. 41(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuhiro Shimonaka: "Changes in the shape of Escherichia coli and human erythrocytes induced by antimicrobial peptides:polymyxin B,EM 49,gramicidin S and tachyplesin" Peptide Chemistry 1994. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takashi katsu: "Ion-selective electrode for procainamide determination in blood serum" Analytica Chimica Acta. (in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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