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Interaction of Bifidobacterium and colonic mucin via carbohydrate-lectin interaction

Research Project

Project/Area Number 06672170
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

YAMAMOTO Kazuo  The University of Tokyo, Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (20174782)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordslectin / Bifidobacterium / mucin
Research Abstract

To determine the specific interactions between Bifidobacterium and colonic mucin, I focused on the interaction of carbohydrate-binding protein (s) expressed on the bacterial cells and colonal mucins. I scanned the ability of B.adolescentis strain Ba56, which were normal inhabitant of the human adult colon, to adhere to the immobilized mucosal components. B.adlescentis was shown to adhere to sulfated mucin, which is expressed on the normal colon. The binding of bacterial cells to sulfated mucin was inhibited by galactose and factose. From the cell surface components of B.adlescentis cells, 44kDa and 30kDa proteins were purified by using affinity chromatography on a column of galactose-Sepharose 4B.The purfied 44 kDa and 30 kDa proteins specifically bound to sulfated mucin expressed on LS174T cells. Benzyl-GalNAc treatment or anti-sulfated mucin monoclonal antibody 91.9H treatment of LS174T cells abolished the binding of ^<125>I-labeled 44kDa and 30kDa proteins. Purified 44 kDa was digested with endoproteinase Lys-C and its proteolytic fragments were recovered by reversed phase HPLC.Partial amino acid sequences of these fragments were analyzed by use of gas-phase protein sequencer.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 山本一夫: "Interaction of immobilized recombinant mouse C-type lectin with glycopeptides and oligosaccharides" Biochemistry. 33. 8159-8166 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 山本一夫: "Cloning and sequence analysis of the Maackia amurensis hemagglutincin cDNA" Glycoconjugate J.11. 572-575 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 河上恭子: "Dual function of macrophage Galactose/N-acetyl galactosamine specific lectins: Glycoprotein up take and tumoricidal recoghition" Jap. J. Cancer Res.85. 744-749 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 山本一夫: "Analysis of aspargine-linked oligosaccharides by sequential lectin affinity chromatography" Molecular Biotechnol.3. 25-36 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小浪悠紀子: "A putative carbohydrate-binding domain of the lactose-binding Cytisus sessilifolius anti-H(o) lectin" Glycoconjugate J.12. 128-134 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小浪悠紀子: "A high degree of sequence homology in the putative carbohydrate recognition domains of Pokeweed mitogens and wheat germ agglutiuin" Glycobiology. 5. 663-670 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 鈴木詔子: "Molecular cloning and expression of cDNA encoding human macrophage C-type lectin" J. Immunol.(印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Yamamoto, C.Ishida, Y.Shinohara, Y.Hasegawa, Y.Konami, T.Osawa, T.Irimura.: "Interaction of immobilized recombinant mouse C-type macrophage lectin with glycopeptides and oligosaccharides." Biochemistry. 33. 8159-8166 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kawakami, K.Yamamoto, S.Toyoshima, T.Osawa, T.Irimura.: "Dual function of macrophage galactose/N-acetylgalactosamine-specific lectins. Glycoprotein uptake and tumor cell recognition." Jap.J.Cancer Res.85. 744-749 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Yamamoto, C.Ishida, Y.Konami, T.Osawa, T.Irimura.: "Cloniong and sequence analysis of the Maackia amurensis hemmagglutinin cDNA." Glycoconjugate J.11. 572-575 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Yamamoto, T.Tsuji, T.Osawa: "Analysis of asparagine-linked oligosaccharides by sequenctial lectin affinity chiromatography." Molecular Biotechnol. 3. 25-36 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Konami, K.Yamamoto, T.Osawa, T.Irimura.: "A puutative carbohydrate-binding domain of the lacotse-binding Cytisus sessilifolius anti-H (O) lectin has a similar amino acid sequence to that of the fucose-binding Ulex euuropaeus anti-H (O) lectin." Glycoconjugate J. 12. 128-134 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Konami, T.Uno, M.Fujii, K.Yamamoto, T.Osawa, T.Irimura: "A high degree of sequence homology in the putative carbohydrate recognition domains of pokeweed mitogen and whieat germ agglutinin : poly-N-acetyllactosamine-binding lectins from different species." Glycobiology. 5. 663-670 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Suzuki, K.Yamamoto, S.Toyoshima, T.Irimura: "Molecular cloning and expression of cDNA encoding human macrophage C-type lectin : Its unique carbohydrate binding specifically for Tn antigen." J.Immunol. (in the press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 山本一夫: "Analysis of asparogine-linked oligosaccharides by sequential lectin affinity chromatography" Molecular Biotechnol.3. 25-36 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小浪悠紀子: "A putative carbohydrate-binding domain of the lactose-binding Cytisus sessilifolius antiH(O) lectin" Glycoconjugate J.12. 128-134 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小浪悠紀子: "A high degree of sequence homology in the putative carbohydrate recognition domains of Pokeweed mitogen and wheat germ agglutinin" Glycobiology. 5. 663-670 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鈴木詔子: "Molecular croning and expression of cDNA encoding human macrophage c-type lectin" J.Immunol. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 山本一夫: "Interaction of immobilized recombinant mouse C-type lectin with siycopeptides and oligosaccharides" Biochemistry. 33. 8159-8166 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 山本一夫: "Cioning and sequence analysis of the Maockia amurensis hensgglutinin cDNA" Glycoconjugate J.11. 572-575 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 河上恭子: "Dual function of macrophoge Galactose/N-acetylgalac Tosumim-specific Lectins:Glycoprotein uptade and Tumericidalrewgniton" Jap.J.Cancer Res.85. 744-749 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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