| Project/Area Number |
06672223
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | The Institute of Physical & Chemical Research |
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Principal Investigator |
HIRABAYASHI Yoshio The Institute of Physical & Chemical Research, Them leader, 国際フロンティア, 研究員 (90106435)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | ganglioside / sialic acid / new / sphingolipid / acetyl-CoA / スフィンゴ脂質 / Ganglioside(ガングリオシド) / モノクローナル抗体 / コリナ-ジックニューロン / プルキンエ細胞 |
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| Research Abstract |
Gangliosides are sialic acid-containing glycosphingolipids present in highest concentrations in the CNS.More than 100 species of gangliosides including extremely minor components exist in the CNS tissues.We found that the minor species including cholinergic neuron specific gangliosides and polysialogangliosides with O-acetylated sialic acid are expressed as neuron type-specific manner. For example, GD1alpha is specifically expressed in the Purkinje neuron in cerebellum.Also, O-acetylgangliosides are present on the molecular layr of cerebellar neurons.Cell biological studies using monoclonal antibodies and metabolic inhibitors have revealed that these unique lipids play roles in variety of cellular processes of neurons including dendritic development and calcium homeostasis.
To understand the biological meaning of O-acetylgangliosides at the molecular-genetical level, we tried to clone a cDNA encoding a factor involved in the formation of O-acetylated gangliosides using expression cloning technology.We have successfully cloned a cDNA coding for one of factors.The isolated clone proved that the protein is totally novel.
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