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Physiological and Biochemical Assessment of Membrane Barrier Function in Inflammatory Disease

Research Project

Project/Area Number 06672280
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用薬理学・医療系薬学
Research InstitutionFaculty of Pharmaceuticla Sciences, Science University of Tokyo

Principal Investigator

HAYASHI Masahiro  Sciences, Science Univrsity of Tokyo Faculty of Pharmaceutical, Professor, 薬学部, 教授 (20012669)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsInflammatory Bowel Disease Model / Membrane Resistance / Short Circuit Current / Damage Score / Electrophysiology / Membrane Permeability / Paracellular Pathway / 粘膜炎症 / 免疫抑制酸性蛋白 / 膜電位
Research Abstract

Membrane barrier function and transport ability for electrolytes were assessed in inflammatory bowel disease. For the inflammatory rat models prepared with trinirobenzene sulfonic acid or acetic acid, relation between the electro-physiological parameter, membrane potential difference, short circuit current (Isc), or membrane resistance (Rm), and the degree of morphological inflammation (damage score) was compared. The parameters decreased with increase in the scores, but the correlation was not significant, resulting that the electro-physiological analysis was considered more objective and accurate. For the acetic acid-models, the decreased Rm recovered to the control value in 4 days after the preparation and the increased permeability of FITC-dextran (FD-4) recovered to the control in 7 days. Accordingly, it was shown that recovery from the disease in the relatively short term can be assessed by the above method. The increase in the permeability of FD-4 and the decrease in Rm suggested the enlargement of the paracellular pathway. The membrane damage by diclofenac sodium (DC) was assessed by the above method. Release of cellular protein was found after perfusion of DC in the rat. However, Isc was increased by the presence of theophylline, suggesting that the membrane metabolizing ability remained normal. In conclusion, the application of the above method is useful in the quantitative assessment of various inflammatory disease.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 熊谷 博,他: "各種大腸炎モデルにおける粘膜機能評価" 消化と吸収. 17. 113-115 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikio Tomita,et al.: "Absorption-Enhancing Mechanism of Sodium Caprate and Decanyolcarnitine in Caco-2 Cells" J.Pharmacol.Exp.Therap.272. 739-743 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroshi Kumagai et al.: "Assessment of Mucosal Function in Some Experimental Colitis Models" Digestion & Absorption. 17 (2). 113-115 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikio Tomita et al.: "Absorption-Enhancing Mechanism of Sodium Caprate and Decanyl-carnitine in Caco-2 Cells" J.Pharmacol. Exp. Therap.272 (2). 739-743 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikio Tomita et al.: "Absorption-Enhancing Mechanism of Sodium Caprate and Decanylcarnitine in Caco-2 Cells" J. Pharmacol. Exp. Therap.272. 739-743 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 熊谷博: "各種大腸炎モデルにおける粘膜機能評価" 消化と吸収. 17. 113-115 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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