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STUDIES ON A BLOOD-GROUP DEPENDENT GLYCOPROTEIN ASSOCIATED SPECIFICALLY WITH HUMAN INTESTINAL ALKALINE PHOSPHATASE

Research Project

Project/Area Number 06672302
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionSAITAMA MEDICAL SCHOOL

Principal Investigator

KOMODA Tsugikazu  SAITAMA MEDICAL SCHOOL,SCHOOL OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (10049835)

Co-Investigator(Kenkyū-buntansha) KOYAMA Iwao  SAITAMA MEDICAL SCHOOL,JUNIOR COLLEGE,ASSOCIATE PROFESSOR, 臨床検査科, 助教授 (30153688)
SAKAGISHI Yoshikatsu  SAITAMA MEDICAL SCHOOL,SCHOOL OF MEDICINE,FULL PROFESSOR, 医学部, 教授 (90049768)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsALKALINE PHOSPHATASE / HIGH-MOLECULAR WIGHT INTESTINAL AP / BLOOD GROUP DEPENDENT / BINDING GLYCOPROTEIN / MOLECULAR SIZE / BLOOD GROUPS B OR O / LEWIS SECRETARY GROUP / B-TRANSFERASE / 小腸アルカリ性ホスファーゼ / O-グリコシド結合型糖鎖 / 絶食時 / 特異的結合タンパク / 精製法 / Triton X-100
Research Abstract

ABNORMALLY MIGRATING ALKALINE PHOSPHATASES (AP_2) ARE DETECTED MORE FREQUENTRY BY POLYACRYLAMIDE GEL DISC ELECTROPHORESIS (PAGE) THAN WITH CELLULOSE ACETATE MEMBRANE,A HIGH-MOLECULAR WEIGHT INTESTINAL AP (HIAP) DETECTED IN HEALTHY SERUM AND ASSOCIATED WITH THE ABH AND LEWIS BLOOD GROUPS WAS INVESTIGATED.BY PREINCUBATION OF BLOOD GROUP B OR O SERA AND A MONOCLONAL ANTIBODY TO HUMAN INTESTINAL AP,THE HIAP WAS FOUND AT THE ORIGIN OF PAGE.IT WAS ABLE TO NORMALIZE HIAP BY BRIEF TREATMENT WITH SUBTILISIN OR PAPAIN.THE RESULTING AP WAS EXHIBITED TO DETECT THE SAME MOBILITY AS COMMON INTESTINAL AP.BLOOD APPEARANCE OF HIAP WAS SPECIFICALLY DEPENDED ON HEALTHY BLOOD GROUP O AND B SUBJECTS BEARING WITH LEWIS b BLOOD GROUP (SECRETARY TYPE). WHEN THE TESTED SUBJECTS BEARING LEWIS a BLOOD GROUP (NON-SECRETARY TYPE) WAS USED,THERE IS LITTLE IF ANY DETECTED THE HIAP IN THEIR CIRCULATIONS.
BASED ON THE RESULTS OF GEL FILTRATION,THE MOLECULAR SIZE OF THE GLYCOPROTEIN BINDING TO INTESTINAL AP HAD APPROXIMATELY 30 KILODALTONS WITHOUT THE INTESTINAL AP AND A DETERGENT.HOWEVER,THE GLYCOPROTEIN BOUND TO INTESTINAL AP IN THE PRESENCE OF TRITONX-100 WAS FOUND AT THE SAME MOBILITY AS THE HIAP IN BLOOD GROUP O AND B SUBJECTS BEARING LEWIS b BLOOD GROUP.FURTHERMORE,IT IS INTERESTING THAT CIRCULATING AMOUNTS OF THE GLYCOPROTEIN BINDING TO INTESTINAL AP ARE MATCHED APPARENTLY TO THE BLOOD GROUP B OR O-TRANSFERASE.
TAKEN TOGETHER,THE BLOOD APPEARRANCE OF INTESTINAL AP IN HUMAN SUBJECTS MAY BE RELATED STRONGLY TO THE BLOOD GROUPS ABH AND LEWIS.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Yamagishi,F.,Komoda,T.,Alpers,D. H. et al: "Clearance of surfactant-like particle proteins from circulation in rats" American Journal of Physiology. 266. G596-G605 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamagishi,F.,Komoda,T.,Alpers,D. H. et al: "Secretion and distribution of rat intestinal surfactant-like particles after fat feeding" American Journal of Physiology. 266. G944-G952 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koyama,I.,Komoda,T.,Sakagishi,Y. et al.: "Carbohydrate-mediated recognition of a circulating placental alkaline phosphatase-immunoglubulin M complex" Clinica Chimica Acta. 230. 9-19 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi,F.,Ikeda,T.,Tozuka,S. et al.: "A variant alkaline phosphatase found in a case of gastric carcinoma with super bone scan" Gut. 36. 299-302 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hoshino,T.,Kumasaka,K.,Kawano,K. et al.: "Low serum alkaline phosphatase activity associated with severe Wilson's disease" Clinica Chimica Acta. 238. 91-100 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 菰田二一,小山岩雄,藤盛葉子,松下誠,入野勤: "小腸様ALPの検出法" 検査と技術. 23. 1039-1045 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 菰田二一,小山岩雄,三浦雅一,中島孝則: "日本臨床(増刊号 広範囲血液・尿化学検査免疫学的検査(上巻)" 日本臨床社(分担執筆), 6 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 菰田二一,小山岩雄: "日本臨床(特集:アイソザイム)" 日本臨床社(分担執筆), 8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] YAMAGISHI,F., BECICH,M.J., EVANS,B.A., KOMODA.T., ALPERS,D.H.: "CLEARANCE OF SURFACTANT-LIKE PARTICLE PROTEINS FROM CIRCULATION IN RATS" AMERICAN JOURNAL OF PHYSIOLOGY. 266. G596-G605 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] YAMAGISHI,F., KOMODA.T., ALPERS,D.H.: "SECRETION AND DISTRIBUTION OF RAT INTESTINAL SURFACTANT-LIKE PARTICLES AFTER FAT FEEDING" AMERICAN JOURNAL OF PHYSIOLOGY. 266. G944-G952 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] KOMODA.T., KOYAMA,I., FUJIMORI,Y., SAKAGISHI,Y., YAMAGISHI,F., HIRANO,K., HIROTA,N.: "A POSSIBLE STRUCTURE OF INTESTINAL-LIKE ALPs AND ITS CLINICAL APPLICATION" JAPANESE JOURNAL OF ELECTROPHORESIS. 38(in Japanese). 167-171 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] KOYAMA,I., SATO,M., SAKAI,H., NAGATA,A., MIURA,M., SAKAGISHI,Y., KOMODA.T.: "CARBOHYDRATE-MEDIATED RECOGNITION OF A CIRCULATING PLACENTAL ALKALINE PHOSPHATASE-IMMUNOGLOBULIN M COMPLEX" CLINICA CHIMICA ACTA. 230. 9-19 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] KOMODA.T.: "RECENT PREGRESS OF THE STUDIESON ALKALINE PHOSPHATASE ISOZYMES AND ITS APPLICATION FOR RENAL DISEASES" RENAL FAILURES. 6(in Japanase). 13-20 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] KOBAYASHI,F., IKEDA,T., TOZUKA,S., NOGUCHI,O., FUKUMA,T., SAKAMOTO,S., MARUMO,F., KOMODA.T., SAKAGISHI,Y., SATO,C.: "A VARIANT ALKALINE PHOSPHATASE FOUND IN A CASE OF GASTRIC CARCINOMA WITH SUPER BONE SCAN" GUT. 36. 299-302 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] HOSHINO,T., KUMASAKA,K., KAWANO,K., YAMAGISHI,KOYAMA,I., FUJIMORI-ARAI,Y., NAKAJIMA,T., KOMODA.T.: "LOW SERUM ALKALINE PHOSPHATASE ACTIVITY ASSOCIATED WITH SEVERE WILSON'S DISEASE" CLINICA CHIMICA ACTA. 238. 91-100 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] ARAI,K.SUMI,S.H., YSHIDA,K., KOMODA.T.: "A PRECURSOR FORM OF HUMAN KIDNEY GAMMA-GLUTAMYL TRANSFERASE IN NORMAL AND CANCEROUS TISSUES,AND ITS POSSIBLE POST-TRANSLATIONAL MODIFICATION" BIOCHIMCA ET BIOPHYSICA. 1253. 33-38 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] KOMODA.T., KOYAMA,I., FUJIMORI,Y., MATSUSHITA,M., IRINO,T.: "SELECTIVE DETECTION OF HUMAN INTESTINAL-LIKE ALKALINE PHOSPHATASE" LABORATORY MEDICINE AND ITS APPLICATIONS. 23(in Japanase). 1039-1045 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakai,H., Yamagishi,F., Miura,M. et al.: "Sugar chain heterogeneity of human urinary chorionic gonadotropin determined by serial lectin affinity chromatography" Tumor Biology. 15. 230-235 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kobayashi,F., Ikeda,T., Tozuka,S. et al.: "A variant alkaline phosphatase found in a case of gastirc carcinoma with super bone scan" Gut. 36. 299-302 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hoshino,T., Kumasaka,K., Kawano,K. et al.: "Low serum alkaline phosphatase activity associated with severe Wilson's disease" Clinica Chimica Acta. 238. 91-100 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Arai,K., Sumi,S., Yoshida,K., Komoda,T.: "A precursor from of human kidney γ-glutamyl transferase in normal and cancer tissues, and its post-translational modification" Biochimica et Biophysica Acta. 1253. 33-38 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 菰田二一,小山岩雄,藤盛葉子,松下誠,入野勤: "小腸様ALPの検出法" 検査と技術. 23. 1039-1045 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 菰田二一,小山岩雄: "特集:アイソザイム(分担)" 日本臨床社, 8 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamagishi,F.,Komoda,T.,Alpers,D.H.et al.: "Clearance of surfactant-like particle proteins from circulation in rats." Am.J.Physiol.266. G596-G605 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamagishi,F.,Komoda,T.,Alpers,D.H.: "Secretion and distribution of rat intestinal surfactant-like particles after fat feeding." Am.J.Physiol.266. G944-G952 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 菰田二一,他: "小腸様ALPの構造と臨床応用" 生物物理化学. 38. 167-171 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 渡辺伸一郎,菰田二一,他: "消化器疾患における高分子型ALPの臨床的意義" 生物物理化学. 38. 161-166 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Koyama,I.,Komoda,T.,Sakagishi,Y.,et al.: "Carbohydrate-mediated recognition of a circulating placental alkaline phosphatase-immunoglobulin M complex." Clin.Chim.Acta. 230. 9-19 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 菰田二一: "アルカリ性ホスファターゼ(ALP)研究の現状と腎疾患への応用" 腎不全. 6. 13-20 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 菰田二一,他: "日本臨床(増刊号広範囲血液・尿化学検査、免疫学的検査)" 尿中アルカリ性ホスファターゼ, 6 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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