Analysis for the reaction mechanism of amylase

Research Project

Project/Area Number	06672303
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Laboratory medicine
Research Institution	Kitasato University School of Allied Health Sciences
Principal Investigator	OGAWA Zensuke Kitasato University, School of Allied Health Sciences, Department of Clinical Chemistry, Lecturer, 医療衛生学部, 講師 (50152385)
Co-Investigator(Kenkyū-buntansha)	ITOH Hajime Kitasato University, School of Health Sciences, Department of Clinical Chemistry, 医療衛生学部, 教授 (10104564)
Project Period (FY)	1994 – 1995
Project Status	Completed (Fiscal Year 1995)
Budget Amount *help	¥2,200,000 (Direct Cost: ¥2,200,000) Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000) Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords	reaction mechanism / amylase
Research Abstract	Recently, structure and reaction mechanism of amylase was known by the X-ray analysis and DNA analysis. Many researcher reported that histidine, aspartic acid, glutamic acid and lysine residue of catalytic center playd an important role for the hydrolysis reaction. We thought the most important role was the recognition of substrate by histidine residue (His). Then, we found out that His modified amylase by diethylpyrocarbonate lost the catalytic activity and this lost activity was recover by the addition of NaN_3. We could suppose the reaction mechanism of hydrolysis mechanism by amylase.