| Project/Area Number |
06680091
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
体育学
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| Research Institution | Niigata University |
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Principal Investigator |
SUGIMOTO Hideo Niigata University, Faculty of Education, Professor, 教育学部, 教授 (20092702)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Toyoji Niigata Cancer Center Hospital, Section of Clinical Chemistry, Section Head, 臨床検査部, 部長
YAMAMOTO Masaharu Niigata University, School of Medicine, Professor, 医学部, 教授 (40018693)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | sports-related stress markers / urinary protein components / alkaline phosphatase / 尿成分 / アルカリフォスファターゼ / 高分子アルカリフォスファターゼ / PAGE / 運動 / 過剰運動 / 成長期 |
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| Research Abstract |
In order to gain a fuller understanding of soprts-related stress markers, we studied urinary protein components and alkaline phosphatase (ALP) levels and isozyme patterns before and after strenous exercise. The urinary protein concentrations and ALP activity-peaks were seen 5-15 minutes and 1 hour after running. SDS-PAGE and Western blotting showed that albumin was the main protein band in the urine which was collected before and 3 houes after running. However, alpha2-macrogloblin, immunogloblin G and transferrin wer seen in the peak frction of total protein concentration, in addition to albumin. While levels of ALP of higher molecular mass were observed to increase slightly, following intense exerclse, neither the intensity nor the duration of the exercise appeared to have an overly significant effect on them.
After buthanol extraction, a distinct band of ALP activity was observed in the polyacrylamide gel, having the same degree of mobility as those of the renal cortex and medulla.
A portion of post-exercise urinary ALP was considered to have originated from the kidney. As ALP is now understood to be one of the markers of bone metabolism, urinary ALP will most certainly come under ever more intense scrutiny, as regards its connection to sports-related stress and osteoporosis.
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